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| Status |
Public on Mar 06, 2007 |
| Title |
CD133+ and CD133- CSC lines in primary human GBM |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We cultured tumor cells from 22 GBM under medium conditions favoring the growth of neural stem cells. 11 out of 15 primary GBM contained a significant CD133+ subpopulation that comprised cells showing all hallmarks of neural stem cells. Cell lines derived from these CD133+ GBM showed a neurosphere-like, non-adherent growth pattern. In contrast, 4 out of 15 cell lines derived from primary GBM grew adherent in vitro and were driven by CD133- tumor cells that fulfilled stem cell criteria. In vivo, these GBM were characterized by a significantly lower proliferation index but similar GFAP staining as compared to CD133+ GBM. Gene arrays from 2x3 representative cells lines are given.
Keywords: Cancer stem cell, CD133, glioblastoma
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| Overall design |
Human glioblastoma cells cultured in DMEM supplemented with EGF, FGF, LIF, B27.
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| Contributor(s) |
Beier CP, Beier D, Bogdahn U, Oefner PJ |
| Citation(s) |
17483311 |
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| Submission date |
Mar 03, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Christoph P. Beier |
| Organization name |
University of Regensburg
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| Department |
Neurology
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| Lab |
NeuroOncology
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| Street address |
Universitatsstrasse 84
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| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA98331 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7181_RAW.tar |
31.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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