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| Status |
Public on Jan 01, 2016 |
| Title |
Microarray analysis of the deletion of transcription regulator SurR in the hyperthermophilic archaeon Thermococcus kodakarensis |
| Organism |
Thermococcus kodakarensis |
| Experiment type |
Expression profiling by array
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| Summary |
Thermococcus kodakarensis preferentially utilizes amino acids as carbon and energy sources in the presence of elemental sulfur as a terminal electron acceptor, while it can assimilate and grow on starch or pyruvate using proton as a terminal acceptor, generating hydrogen in the absence of elemental sulfur. SurR is a transcriptional regulator controlling hydrogen and elemental sulfur metabolism. To identify the genes that are under the regulation of Tk-SurR, we investigated the transcriptional profiling of Tk-SurR deletion strain grown in the presence of elemental sulfur at 85˚C by comparing with the host strain, KU216.
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| Overall design |
One-condition experiment, KU216 vs. DTS cells. Technical replicates: 2 DTS grown in the presence of elemental sulfur at 85˚C, independently measured. One replicate per array.
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| Contributor(s) |
Yamashita K, Hidese R, Fujiwara S, Kanai T, Atomi H, Imanaka T |
| Citation missing |
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| |
| Submission date |
Aug 12, 2015 |
| Last update date |
Jan 03, 2016 |
| Contact name |
Ryota Hidese |
| E-mail(s) |
rhidese0830@gmail.com
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| Organization name |
Kwansei-Gakuin University
|
| Department |
School of Science and Technology
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| Lab |
Fujiwara lab.
|
| Street address |
Gakuen 2-1
|
| City |
Sanda |
| State/province |
Hyogo |
| ZIP/Postal code |
669-1337 |
| Country |
Japan |
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| Platforms (1) |
| GPL17565 |
Thermococcus kodakarensis microarray |
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| Samples (2) |
| GSM1849295 |
DTS in the presence elemental sulfur, replicate 1 |
| GSM1849296 |
DTS in the presence elemental sulfur, replicate 2 |
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| Relations |
| BioProject |
PRJNA292662 |
