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| Status |
Public on Mar 09, 2007 |
| Title |
Cytokine treated normal human epidermal keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Normal human epidermal keratinocytes (NHEK) from neonatal foreskin were cultured in serum-free EpiLife medium with human KC growth supplement (0.2% bovine pituitary extract (v/v), 5ug bovine insulin, 5ug/ml bovine transferrin, 0.5ng/ml human EGF, and 0.18 ug/ml hydrocortisone) from Cascade Biologics. Cultures were treated with recombinant cytokines from R&D Systems. J Immunol. 2007 Feb 15;178(4):2229-40.
Keywords: cytokine response
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| Overall design |
NHEK were treated with IL-19, IL20, IL-22, and IL24, with controls untreated, along with IL1b, IFN gamma, and KGF.
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| Contributor(s) |
Sa SM, Valdez PA, Wu J, Jung K, Zhong F, Hall L, Kasman I, Winer J, Modrusan Z, Danilenko DM, Ouyang W |
| Citation(s) |
17277128 |
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| Submission date |
Mar 06, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Kenneth Jung |
| E-mail(s) |
kjung@gene.com
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| Organization name |
Genentech
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| Street address |
1 DNA Way MS93
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94122 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (25)
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| GSM173535 |
Treated with IL19 PRB11898 (HGU133P) |
| GSM173536 |
Treated with IL19 PRB11899 (HGU133P) |
| GSM173537 |
Treated with IL19 PRB11900 (HGU133P) |
| GSM173538 |
Treated with IL20 PRB11901 (HGU133P) |
| GSM173539 |
Treated with IL20 PRB11902 (HGU133P) |
| GSM173540 |
Treated with IL20 PRB11903 (HGU133P) |
| GSM173541 |
Treated with IL22 PRB11904 (HGU133P) |
| GSM173542 |
Treated with IL22 PRB11905 (HGU133P) |
| GSM173543 |
Treated with IL22 PRB11906 (HGU133P) |
| GSM173544 |
Treated with IL24 PRB11907 (HGU133P) |
| GSM173545 |
Treated with IL24 PRB11908 (HGU133P) |
| GSM173546 |
Treated with IL24 PRB11909 (HGU133P) |
| GSM173547 |
Treated with IL26d PRB11910 (HGU133P) |
| GSM173548 |
Treated with IL26d PRB11911 (HGU133P) |
| GSM173549 |
Treated with IL26d PRB11912 (HGU133P) |
| GSM173550 |
Treated with KGF PRB11913 (HGU133P) |
| GSM173551 |
Treated with KGF PRB11914 (HGU133P) |
| GSM173552 |
Treated with KGF PRB11915 (HGU133P) |
| GSM173553 |
Treated with IFNg PRB11916 (HGU133P) |
| GSM173554 |
Treated with IFNg PRB11917 (HGU133P) |
| GSM173555 |
Treated with IL1b PRB11919 (HGU133P) |
| GSM173556 |
Treated with IL1b PRB11920 (HGU133P) |
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| Relations |
| BioProject |
PRJNA98301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7216_RAW.tar |
130.3 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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