|
| Status |
Public on Aug 20, 2015 |
| Title |
Expression data from The human glioblastoma cell culture resource: validated cell models representing all molecular subtypes (transcript) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To explore the degree to which the glioma cell lines remained transcriptionally stable under diverse experimental conditions, we transplanted three different lines (U3020MG, U3047MG and U3065MG) intracranially to NOD-SCID mice; explanted the resulting tumors and cultured the cells for two passages, and then isolated RNA from the cell line prior to transplantation (U3020MG-p10, U3047MG-p7, U3065MG-p10), from the xenograft tumor and from the explanted cells.
|
| |
|
| Overall design |
Total RNA extracted from both tumor tissues and glioma cell lines using the miRNeasy Mini kit (Qiagen) was labeled and hybridized on Affymetrix GeneChip Human Transcriptome Array 2.0. Expression levels were RMA-normalized employing the Affymetrix Expression Console software.
|
| |
|
| Contributor(s) |
Nelander S, Uhrbom L |
| Citation(s) |
26629530 |
| Submission date |
Aug 19, 2015 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Sven Nelander |
| Organization name |
Uppsala Universitet
|
| Street address |
Rudbecklab
|
| City |
Uppsala |
| ZIP/Postal code |
75217 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
|
| Samples (15)
|
|
| This SubSeries is part of SuperSeries: |
| GSE72219 |
The human glioblastoma cell culture resource |
|
| Relations |
| BioProject |
PRJNA293383 |
Less...
More...