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Status |
Public on Aug 21, 2015 |
Title |
Preadult life history variation determines adult transcriptome expression |
Organism |
Drosophila mojavensis |
Experiment type |
Expression profiling by array
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Summary |
Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate preadult life history affects over adult transcriptome expression.
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Overall design |
A total of 137 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and four "length of egg to adult development time" points, i.e. 14, 15, 16, and 17 days. (14 days:D1; 15 days:D2; 16 days:D3; 17 days:D4). Each chip measures the expression level of 14528 transcripts. One to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.
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Contributor(s) |
Gibbs AG, Etges WJ, Rajpurohit S, de Oliveira CC |
Citation(s) |
26615085 |
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Submission date |
Aug 20, 2015 |
Last update date |
Feb 24, 2016 |
Contact name |
Allen G. Gibbs |
E-mail(s) |
allen.gibbs@unlv.edu
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Phone |
702-895-3203
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Organization name |
University of Nevada
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Department |
School of Life Sciences
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Lab |
Gibbs Lab
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Street address |
4505 S Maryland Parkway
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City |
Las Vegas |
State/province |
Nevada |
ZIP/Postal code |
89154 |
Country |
USA |
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Platforms (1) |
GPL16433 |
NimbleGen Gibbs Drosophila mojavensis 135K 12-plex Array |
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Samples (137)
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Relations |
BioProject |
PRJNA293445 |