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Series GSE72255 Query DataSets for GSE72255
Status Public on Feb 10, 2016
Title Expression data from fully-expanded leaflet of Bambara groundnut genotype (S19-3)
Platform organism Glycine max
Sample organism Vigna subterranea
Experiment type Expression profiling by array
Summary Low temperature stress in a number of African countries, such as Botswana, at night can effect the growth and development of bambara groundnut, leading to losses in potential crop yield. Therefore, we made an attempt to identify and analyze the genes and gene modules associated with low temperature stress response in bambara groundnut using the cross-species microarray technique, as bambara groundnut has no microarray chip, coupled with network-based analysis.
 
Overall design Three plants of the bambara groundnut genotype ‘S19-3’ were grown in controlled environment growth rooms at Sutton Bonington Campus, University of Nottingham under a 12 hour photoperiod and at a constant temperature of 27°C. Plants were grown in soil columns containing a growing medium of 1 part John Innes 2 compost to 1 part sand, and were watered as required. A single, fully-expanded leaflet was sampled from each of the three plants growing at 27°C, snap frozen in liquid nitrogen and stored at -80°C. Plants were given a further 3 days at 27°C to recover from sampling before being moved to a controlled environment room at 23°C. On the fifth day at 23°C a single fully-expanded leaflet was sampled and stored, as described above. After 3 further days plants were moved to 18°C, and then five days later were sampled again.
 
Contributor(s) Bonthala V, Mayes K, Mayes S, Massawe F, Twycross J
Citation(s) 26859686
Submission date Aug 20, 2015
Last update date Nov 22, 2016
Contact name Venkata Suresh Bonthala
E-mail(s) psxvb@nottingham.ac.uk
Organization name University of Nottingham
Department School of Computer Science
Street address Wollaton Road
City Nottingham
ZIP/Postal code NG8 1PA
Country United Kingdom
 
Platforms (1)
GPL4592 [Soybean] Affymetrix Soybean Genome Array
Samples (10)
GSM1858874 Plant at 27°C, biological rep 1
GSM1858875 Plant at 27°C, biological rep 2
GSM1858876 Plant at 27°C, biological rep 3
Relations
BioProject PRJNA293473

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Supplementary file Size Download File type/resource
GSE72255_RAW.tar 40.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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