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| Status |
Public on Apr 02, 2018 |
| Title |
Wound healing process after ventricular resection in zebrafish: time course (zebrafish) |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
The mechanism of cardiomyocyte prliferation and migration after ventricular resection is a complex and dynamic process. To resolve this, we chose the zebrafish model organism for studying post-injury heart regeneration and wound healing progress via systems biology approach. Transcriptome microarray data and histological analysis of injured fish were sampled at different time points during recovery process. Time-course microarray data following wound healing of zebrafish were obatined. From this set of data, we constructed two intracellular protein–protein interaction (PPI) networks to provide insights into the ventricular resection wound healing mechanism.
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| Overall design |
Each fish in each group was injured by ventricular resection from day 0 to 28, respectively. These injured fish were collected at 0, 0.25, 1, 3, 6, 10, 15, 21, 28 dpi (day post injury). 0.625μg of Cy3 cRNA for C. albicans array and 1.65 μg of Cy3 cRNA for zebrafish array was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 minutes. Each time points contain two biological repeats.
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| Contributor(s) |
Chuang Y, Lin C, Chen B, Hsieh W, Wong DH |
| Citation(s) |
29560825 |
| |
| Submission date |
Aug 25, 2015 |
| Last update date |
May 06, 2018 |
| Contact name |
Yung-Jen Chuang |
| E-mail(s) |
yjchuang@life.nthu.edu.tw
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| Phone |
886-3-574-2764
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| Organization name |
National Tsing Hua University
|
| Department |
Department of Medical Science
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| Lab |
YJ Chuang
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| Street address |
No. 101, Sec. 2, Kuang Fu Road
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| City |
Hsinchu |
| State/province |
Taiwan |
| ZIP/Postal code |
30013 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6457 |
Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Feature Number version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA293854 |
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