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Status |
Public on Dec 21, 2016 |
Title |
A transcription factor pulse can prime chromatin for heritable transcriptional memory [ChIPseq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
To investigate the epigenetic control of long-term transcriptionnal memory (LTTM) we used a trans-differentiation system where Bcell are reprogramed into macrophages upon induction of the transcription factor Cebpa. We performed ChIPseq in untreated Bcells, after 12h of Cebpa induction and after 6days of “wash” of Cebpa, to see if any proteins/histone marks are retained.
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Overall design |
Genome binding/occupancy of transcription factor Pu1 and histones (H3K4me1/2/3 and H3K27me3) by ChIPseq in uninduced Bcell (Control), after induction Cebpa for 6h (Pulse) and after 6 days of wash (Chase).
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Contributor(s) |
Badeaux A, Collombet S, Graf T, Thieffry D, Shi Y |
Citation(s) |
27920256 |
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Submission date |
Aug 28, 2015 |
Last update date |
May 15, 2019 |
Contact name |
samuel Collombet |
E-mail(s) |
samuelcollombet@gmail.com
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Organization name |
Ecole Normale Superieure
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Department |
Biology
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Lab |
Thieffry
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Street address |
46 rue d'Ulm
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City |
Paris |
ZIP/Postal code |
75005 |
Country |
France |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (17)
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This SubSeries is part of SuperSeries: |
GSE72488 |
A transcription factor pulse can prime chromatin for heritable transcriptional memory |
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Relations |
BioProject |
PRJNA294146 |
SRA |
SRP062944 |