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| Status |
Public on Sep 24, 2019 |
| Title |
MEK and GSK3Ī² inhibition drives ES cells into a naive pluripotent state irrespective of their global methylation statusĀ |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We carry out methyl DNA immunoprecipitation (meDIP), hydroxymethyl DNA immunoprecipitaion (hmeDIP) and H3K27me3 chromatin imminoprecipitation (ChIP) prior to sequencing on the Ion Proton semiconductor sequencing platform to report on the genome-wide epigenetic patterns in J1 mESC and mutant mESCs (hyper active DNMT3b/3l or DNMT1/3a/3b triple knock out) grown under serum conditions or in 2i meadia for 14 days
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| |
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| Overall design |
Genome-wide patterns were generate on the Ion Proton P1 sequencer.
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| |
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| Contributor(s) |
Thomson JP, Shukla R, Mjoseng H, Fawkes A, Clark R, Murphy L, Meehan RR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 31, 2015 |
| Last update date |
Jul 25, 2021 |
| Contact name |
John Paterson Thomson |
| E-mail(s) |
john.thomson@igmm.ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
MRC Human Genetics Unit
|
| Lab |
Meehan
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA294321 |
| SRA |
SRP063019 |
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