|
| Status |
Public on Nov 17, 2007 |
| Title |
Yeast mRNA decay analysis after addition of 3 µ/mL thiolutin |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Time course after the addition of the transcriptional inhibitor thiolutin at 3µg/mL to an exponential growing culture of S. cerevisiae in YPD.
Keywords: time course, mRNA stability analysis
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| |
|
| Overall design |
Total RNA from BQS252 yeast strain (Mat a, ura 3-52 derived from FY1679) growing in exponential phase in YPD was extracted at different times after thiolutin 3µg/mL addition. All the samples were hybridized on the same nylon array. In order to estimate mRNA stability the intensities where normalized using global poly(A) data from a RNA dot-blot and assuming a first order decaying. There is only 1 replicate.
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| |
|
| Contributor(s) |
Pelechano V, Pérez-Ortín J |
| Citation(s) |
17914747 |
| |
| Submission date |
Mar 14, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Jose E. Perez-Ortin |
| E-mail(s) |
jose.e.perez@uv.es
|
| Phone |
34 963 543467
|
| Organization name |
Universitat de Valencia
|
| Department |
Bioquimica y Biologia Molecular
|
| Lab |
Yeast Functional Genomics (GFL)
|
| Street address |
Dr. Moliner 50
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
E46100 |
| Country |
Spain |
| |
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| Platforms (1) |
|
| Samples (5)
|
| GSM175046 |
BQS252_thiolutin_t0min_replicate 1 |
| GSM175047 |
BQS252 thiolutin t10min replicate 1 |
| GSM175048 |
BQS252 thiolutin t20min replicate 1 |
| GSM175049 |
BQS252_thiolutin_3ug t30min replicate 1 |
| GSM175050 |
BQS252 thiolutin t45min replicate 1 |
|
| Relations |
| BioProject |
PRJNA98017 |
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