|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Sep 05, 2007 |
Title |
Evaluation of murine mast cells derived exosomal RNA versus their parental cells MC/9. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Exosomes are vesicles of endocytic origin released by many types of cells into the extracellular environment. In an attempt to further examine the exosome-mediated cellular communication, we show that exosomes from a mouse mast cell line (MC/9), exosomes from primary bone marrow derived mast cells, and exosomes from a human mast cell line (HMC-1) contain RNA but not DNA. Microarray assessments of exosome-derived RNA revealed that these vesicles contain mRNA from approximately 1200 genes, many of which are unique and not present in the cytoplasmic RNA pool in the donor cell. Keywords: Exosomal RNA versus their parental cells, MC/9
|
|
|
Overall design |
Exosomes were prepared from the supernatant of MC/9 cells by differential centrifugations and filtration. RNA was isolated from the exosomes and their parental cells using Trizo. The microarray experiments were performed by SweGene (www.swegene.org/) according to Affymetrix microarray DNA chip analysis. The experiment was performed in quadruple samples. ExoRNA1, ExoRNA2, ExoRNA3, and ExoRNA4 for the exosomes samples and Mast_cells1, Mast_cells2, Mast_cells3, and Mast_cells4 for the MC/9 cells.
|
|
|
Contributor(s) |
Valadi H, Lötvall J |
Citation(s) |
17486113 |
|
Submission date |
Mar 15, 2007 |
Last update date |
Feb 11, 2019 |
Contact name |
Hadi Valadi |
E-mail(s) |
hadi.valadi@gu.se
|
Phone |
+46(0)704561604
|
Organization name |
University of Gothenburg
|
Department |
Dept. of Rheumatology and Inflammation Research
|
Street address |
Guldhedsgatan 10A
|
City |
Gothenburg |
ZIP/Postal code |
405 30 |
Country |
Sweden |
|
|
Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA97983 |
Supplementary file |
Size |
Download |
File type/resource |
GSE7275_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
Processed data included within Sample table |
|
|
|
|
|