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Series GSE72770 Query DataSets for GSE72770
Status Public on Dec 31, 2015
Title Whole Genome Bisulfite sequencing of Arabidopsis thaliana High and Low phosphate methylomes
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Pi availability is a significant limiting factor for plant growth in both natural and agricultural systems. To cope with such limiting conditions, plants have adapted developmental and biochemical strategies to enhance Pi acquisition and to avoid starvation. A myriad of genes that are involved in the regulation and display of these strategies have been identified. However, the possible epigenetic components regulating the phosphate starvation responses have not been thoroughly investigated. DNA methylation is a major epigenetic mark involved in diverse biological processes and it may play a critical role in Pi starvation stress adaptation, also changes in DNA methylation can lead to a unique gene expression pattern in response to specific developmental and environmental conditions. Here in we demonstrate that non-CpG DNA methylation is required for proper expression of a number of Pi-limitation responsive genes in Arabidopsis thaliana and results in altered morphologic and physiologic phosphate starvation responses.Our data suggest that DNA methylation is involved in the modulation of Pi starvation responses via the transcriptional regulation of a set of phosphate-starvation responsive genes.
 
Overall design Analysis of 8 different treatments, 2 different Organs (Root and Shoot), 2 different Phosphate treatments (High Pi, Low Pi), 2 different Times (Short Term, Long Term), 2 biological replicates for treatment
 
Contributor(s) Yong-Villalobos L, Herrera-Estrella LR, Martinez-de la Vega O
Citation(s) 26668375
Submission date Sep 08, 2015
Last update date May 15, 2019
Contact name Luis Rafael Herrera Estrella
Organization name LANGEBIO CINVESTAV
Department LANGEBIO
Lab Physiology and Metabolic Engineering of Plants
Street address Km 9.6 Libramiento Norte Carretera León
City Irapuato
State/province Guanajuato
ZIP/Postal code 36821
Country Mexico
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (16)
GSM1870018 +Pit Root 7 dat Meth
GSM1870019 +Pit Shoot 7 dat Meth
GSM1870020 -Pit Root 7 dat Meth
Relations
BioProject PRJNA295025
SRA SRP063421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72770_Pi_Starvation_RNASeq_counts_Total.txt.gz 417.7 Kb (ftp)(http) TXT
GSE72770_Plusvsminus_Root16dat.csv.gz 28.6 Kb (ftp)(http) CSV
GSE72770_Plusvsminus_Root7dat.csv.gz 39.2 Kb (ftp)(http) CSV
GSE72770_Plusvsminus_Shoot16dat.csv.gz 60.1 Kb (ftp)(http) CSV
GSE72770_Plusvsminus_Shoot7dat.csv.gz 39.0 Kb (ftp)(http) CSV
GSE72770_RAW.tar 3.1 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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