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Status |
Public on Dec 31, 2015 |
Title |
Whole Genome Bisulfite sequencing of Arabidopsis thaliana High and Low phosphate methylomes |
Organism |
Arabidopsis thaliana |
Experiment type |
Methylation profiling by high throughput sequencing Expression profiling by high throughput sequencing
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Summary |
Pi availability is a significant limiting factor for plant growth in both natural and agricultural systems. To cope with such limiting conditions, plants have adapted developmental and biochemical strategies to enhance Pi acquisition and to avoid starvation. A myriad of genes that are involved in the regulation and display of these strategies have been identified. However, the possible epigenetic components regulating the phosphate starvation responses have not been thoroughly investigated. DNA methylation is a major epigenetic mark involved in diverse biological processes and it may play a critical role in Pi starvation stress adaptation, also changes in DNA methylation can lead to a unique gene expression pattern in response to specific developmental and environmental conditions. Here in we demonstrate that non-CpG DNA methylation is required for proper expression of a number of Pi-limitation responsive genes in Arabidopsis thaliana and results in altered morphologic and physiologic phosphate starvation responses.Our data suggest that DNA methylation is involved in the modulation of Pi starvation responses via the transcriptional regulation of a set of phosphate-starvation responsive genes.
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Overall design |
Analysis of 8 different treatments, 2 different Organs (Root and Shoot), 2 different Phosphate treatments (High Pi, Low Pi), 2 different Times (Short Term, Long Term), 2 biological replicates for treatment
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Contributor(s) |
Yong-Villalobos L, Herrera-Estrella LR, Martinez-de la Vega O |
Citation(s) |
26668375 |
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Submission date |
Sep 08, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Luis Rafael Herrera Estrella |
Organization name |
LANGEBIO CINVESTAV
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Department |
LANGEBIO
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Lab |
Physiology and Metabolic Engineering of Plants
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Street address |
Km 9.6 Libramiento Norte Carretera León
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City |
Irapuato |
State/province |
Guanajuato |
ZIP/Postal code |
36821 |
Country |
Mexico |
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Platforms (1) |
GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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Samples (16)
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Relations |
BioProject |
PRJNA295025 |
SRA |
SRP063421 |