|
| Status |
Public on Nov 25, 2015 |
| Title |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Within the bone marrow, hematopoietic stem cells differentiate and give rise to diverse blood cell types and functions. Currently, hematopoietic progenitors are defined using surface markers combined with functional assays that are not directly linked with the in vivo potential or gene regulatory mechanisms. Here we comprehensively identify myeloid progenitor subpopulations by transcriptional sorting of single cells from the bone marrow. We describe multiple progenitor subgroups showing unexpected transcriptional priming towards seven differentiation fates, but no progenitors with a mixed state. Transcriptional differentiation is correlated with combinations of known and previously undefined transcription factors, suggesting the process is tightly regulated. Histone maps and knockout assays are consistent with the transcriptional states while traditional transplantation experiments are only partially overlapping myeloid transcriptional priming. Our analyses uncover the function of the underlying regulatory mechanisms for several sub groups and establishes a general framework for dissecting hematopoiesis.
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| |
|
| Overall design |
Bone marrow common myeloid progenitor H3K4me2 profiles were generated by deep sequencing of iChIP libraries on an Illumina NextSeq
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| |
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| Contributor(s) |
Amit I, Tanay A, Paul F, Arkin Y, Giladi A |
| Citation(s) |
26627738 |
| |
| Submission date |
Sep 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Amit |
| E-mail(s) |
ido.amit@weizmann.ac.il
|
| Phone |
972-8-9343338
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Immunology
|
| Street address |
234 Herzl st.
|
| City |
Rehovot |
| ZIP/Postal code |
760001 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE72859 |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors |
|
| Relations |
| BioProject |
PRJNA295199 |
| SRA |
SRP063521 |
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