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| Status |
Public on Mar 09, 2016 |
| Title |
Transcriptomic analysis of Primary duck hepatocyte with differential susceptibility |
| Organism |
Anas platyrhynchos |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Our aim was to classify and quantify transcripts in primary duck hepatocytes cultured in medium with 5% FBS or 1.5% DMSO for 8 days.
Methods: The transcriptome of PDHs under different conditions was analyzed by the pair-end sequencing on the Illumina Solexa platform. High-quality reads were mapped to the Anas platyrhynchos genome with TopHat v2.0.12 software. TopHat allows multiple alignments per read and default parameters were used. Cufflinks v2.2.1 software was later used for analyses that included transcript assembly and FPKM value calculations to quantify gene expression; this program was also run with default parameters.
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| |
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| Overall design |
The transcriptome of PDHs under different culture conditions were analyzed by the paired-end sequencing on the Illumina Solexa platform.
|
| |
|
| Contributor(s) |
Yan L, Qu D |
| Citation(s) |
26900848 |
| |
| Submission date |
Sep 09, 2015 |
| Last update date |
Oct 30, 2020 |
| Contact name |
LIANG YAN |
| E-mail(s) |
henshore@163.com
|
| Organization name |
Fudan University
|
| Street address |
NO 131, Dongan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
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| Platforms (1) |
| GPL19739 |
Illumina HiSeq 2000 (Anas platyrhynchos) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA295222 |
| SRA |
SRP063533 |
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