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Status |
Public on Sep 10, 2015 |
Title |
Paracrine stimulation effect of autologous salispheres on mouse salivary gland |
Platform organism |
Mus musculus |
Sample organisms |
Homo sapiens; Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Human salispheres, a culture of stem/progenitor cells, represent a potential therapy for radiation induced hyposalivation. Radiation-induced hyposalivation dramatically reduces quality of life of patients. We have demonstrated the potential of human salispheres to engraft and differentiate when transplanted into a mouse model of hyposalivation, in the manuscript associated with these data. We also demonstrate the functional recovery of irradiated salivary glands (SGs) following human salisphere transplantation, by the measurement of saliva production. We previously employed Illumina microarrays to determine if transplanted human salisphere cells exert a paracrine stimulatory effect on recipient mouse SGs. Results of this array unveiled a large cohort of immuneresponse genes unregulated following human salisphere transplantation. In order to negate this immune response and unveil any true paracrein stimulatory effects, we performed autologous transplantation of salispheres from NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, the same model employed in our first microarray study, in the irradiated SGs of NSG mice.
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Overall design |
6 samples were analysed in total. Total RNA from 3 irradiated control SGs (5Gy irradiation) and 3 salivary glands transplanted with 100,000 NSG salispheres.
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Contributor(s) |
Pringle SA, Zwart E |
Citation missing |
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Submission date |
Sep 09, 2015 |
Last update date |
Jan 16, 2019 |
Contact name |
Sarah Pringle |
E-mail(s) |
s.a.pringle@umcg.nl
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Phone |
0031503638996
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Organization name |
University Medical Centrum Groningen
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Department |
Cell Biology
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Street address |
Antonius Deusinglaan 1
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City |
Groningen |
State/province |
Groningen |
ZIP/Postal code |
9713 AV |
Country |
Netherlands |
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Platforms (1) |
GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE72871 |
Paracrine stimulation effect of autologous salispheres and human salispheres on mouse salivary gland |
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Relations |
BioProject |
PRJNA295220 |