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Series GSE72870 Query DataSets for GSE72870
Status Public on Sep 10, 2015
Title Paracrine stimulation effect of autologous salispheres on mouse salivary gland
Platform organism Mus musculus
Sample organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary Human salispheres, a culture of stem/progenitor cells, represent a potential therapy for radiation induced hyposalivation. Radiation-induced hyposalivation dramatically reduces quality of life of patients. We have demonstrated the potential of human salispheres to engraft and differentiate when transplanted into a mouse model of hyposalivation, in the manuscript associated with these data. We also demonstrate the functional recovery of irradiated salivary glands (SGs) following human salisphere transplantation, by the measurement of saliva production. We previously employed Illumina microarrays to determine if transplanted human salisphere cells exert a paracrine stimulatory effect on recipient mouse SGs. Results of this array unveiled a large cohort of immuneresponse genes unregulated following human salisphere transplantation. In order to negate this immune response and unveil any true paracrein stimulatory effects, we performed autologous transplantation of salispheres from NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, the same model employed in our first microarray study, in the irradiated SGs of NSG mice.
 
Overall design 6 samples were analysed in total. Total RNA from 3 irradiated control SGs (5Gy irradiation) and 3 salivary glands transplanted with 100,000 NSG salispheres.
 
Contributor(s) Pringle SA, Zwart E
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Submission date Sep 09, 2015
Last update date Jan 16, 2019
Contact name Sarah Pringle
E-mail(s) s.a.pringle@umcg.nl
Phone 0031503638996
Organization name University Medical Centrum Groningen
Department Cell Biology
Street address Antonius Deusinglaan 1
City Groningen
State/province Groningen
ZIP/Postal code 9713 AV
Country Netherlands
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (6)
GSM1873677 SG_irradiated control_rep1 reanalysis
GSM1873678 SG_irradiated control_rep2 reanalysis
GSM1873679 SG_irradiated control_rep3 reanalysis
This SubSeries is part of SuperSeries:
GSE72871 Paracrine stimulation effect of autologous salispheres and human salispheres on mouse salivary gland
Relations
BioProject PRJNA295220

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72870_RAW.tar 15.8 Mb (http)(custom) TAR
GSE72870_non-normalized.txt.gz 3.0 Mb (ftp)(http) TXT
GSE72870_normalized.txt.gz 3.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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