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Status |
Public on Nov 09, 2015 |
Title |
Genome-wide DNA methylation change due to lncRNA H19 knockdown in myotubes |
Organism |
Mus musculus |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
We used Methyl-MiniSeq platform from Zymo Research company to identify genome-wide methylation changes affected by lncRNA H19 knockdown in myotubes. Following H19 knockdown, we observed extensive genome-wide mthylation pattern changes relative to siCon cells, with some genes showing incresed methylation, others showing decreased methylation, and a third group with no significant change.
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Overall design |
Myotubes differentiated from mouse C3H myoblasts were transfected with either control siRNA or siH19, 48h later, cellular genomic DNA was extracted and subjected to genome-scale DNA methylation mapping using the platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS).
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Contributor(s) |
Giang K, Chung H, Sun X |
Citation(s) |
26687445 |
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Submission date |
Sep 14, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Na Zhang |
Organization name |
UConn Health
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Department |
Genetics and Genome Sciences
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Lab |
Gordon G. Carmichael
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Street address |
400 Farmington Ave
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City |
Farmington |
State/province |
CT |
ZIP/Postal code |
06030 |
Country |
USA |
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Platforms (1) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE73014 |
Genome-wide DNA-methylation and transcriptome change due to lncRNA H19 knockdown in myotubes |
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Relations |
BioProject |
PRJNA296100 |
SRA |
SRP063884 |