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| Status |
Public on Nov 09, 2015 |
| Title |
Genome-wide DNA methylation change due to lncRNA H19 knockdown in myotubes |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We used Methyl-MiniSeq platform from Zymo Research company to identify genome-wide methylation changes affected by lncRNA H19 knockdown in myotubes. Following H19 knockdown, we observed extensive genome-wide mthylation pattern changes relative to siCon cells, with some genes showing incresed methylation, others showing decreased methylation, and a third group with no significant change.
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| Overall design |
Myotubes differentiated from mouse C3H myoblasts were transfected with either control siRNA or siH19, 48h later, cellular genomic DNA was extracted and subjected to genome-scale DNA methylation mapping using the platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS).
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| Contributor(s) |
Giang K, Chung H, Sun X |
| Citation(s) |
26687445 |
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| Submission date |
Sep 14, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Na Zhang |
| Organization name |
UConn Health
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| Department |
Genetics and Genome Sciences
|
| Lab |
Gordon G. Carmichael
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| Street address |
400 Farmington Ave
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| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE73014 |
Genome-wide DNA-methylation and transcriptome change due to lncRNA H19 knockdown in myotubes |
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| Relations |
| BioProject |
PRJNA296100 |
| SRA |
SRP063884 |
