|
| Status |
Public on Sep 16, 2018 |
| Title |
High-throughput RNAi cell viability screen to identify selective targets for EWS-FLI1 positive Ewing sarcoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have performed a high-throughput RNA interference screen to identify targets inhibiting EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 expressing A673 Ewing sarcoma cells were screened both in presence and absence of EWS-FLI1 shRNA induction with druggable siRNA library. Leucine rich repeats and WD repeat Domain containing 1 (LRWD1) targeting siRNA pool was the strongest anti-proliferative hit identified only in presence of EWS-FLI1. Validation experiments confirmed the anti-proliferative effect of LRWD1 depletion especially in EWS-FLI1 expressing cells. Functional analysis of differentially expressed genes in LRWD1 depleted Ewing sarcoma cells showed over-representation of connective tissue development, cell projection morphogenesis and neuronal processes.
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| |
|
| Overall design |
Examination of the effect of two LRWD1 targeting siRNA molecules vs. scrambled siRNA in one cell line
|
| |
|
| Contributor(s) |
Iljin K, He T |
| Citation(s) |
27760381 |
| |
| Submission date |
Sep 16, 2015 |
| Last update date |
Dec 21, 2021 |
| Contact name |
Kristiina Iljin |
| E-mail(s) |
kristiina.iljin@vtt.fi
|
| Organization name |
VTT
|
| Street address |
Tietotie 2
|
| City |
Espoo |
| ZIP/Postal code |
02044 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (9)
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|
| Relations |
| BioProject |
PRJNA296043 |
| SRA |
SRP063851 |
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