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| Status |
Public on Sep 24, 2015 |
| Title |
Phasevarion of non-typeable Haemophilus influenzae R2866 |
| Platform organism |
Haemophilus influenzae |
| Sample organism |
Haemophilus influenzae R2866 |
| Experiment type |
Expression profiling by array
|
| Summary |
H. influenzae R2866 contains a phase-variable DNA methylase modA10, by virtue of 16 tetrameric repeats present after the start codon. Gain or loss of repeats during replication by slipped-strand mispairing can result in a non-functional reading frame. Microarray analyses of wild-type and insertionally inactivated modA were performed to determine genes affected by phase variable modification.
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| Overall design |
Three replicate cultures were grown in either supplemented brain heart infusion broth (R2866 and R3763) or chemically defined media (R3790 and R3791) to an optical density at 600nm of 0.3 to 0.4 (mid-log phase). RNA was isolated by the method of Jahn et al.
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| Contributor(s) |
VanWagoner TM, Nelson KL, Fox K, Smith HK, Srikhanta YN, Jennings MP, Atack JM, Stull TL, Smith AL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 23, 2015 |
| Last update date |
Sep 26, 2015 |
| Contact name |
Timothy M VanWagoner |
| E-mail(s) |
timothy-vanwagoner@ouhsc.edu
|
| Phone |
405-271-6451
|
| Organization name |
University of Oklahoma Health Sciences Center
|
| Department |
Pediatrics
|
| Lab |
Stull
|
| Street address |
940 SL Young Blvd, BMS340
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
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| Platforms (1) |
| GPL6594 |
Nimblegen custom Haemophilus influenzae multi-genome array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA296784 |
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