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| Status |
Public on Sep 29, 2015 |
| Title |
MicroRNA expression analysis of livers of F1 male offspring fathered from control mice or stressed mice |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Cytomegalovirus; Lymphocryptovirus; Rhadinovirus; JC polyomavirus; Human immunodeficiency virus 1; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Psychological stress is highly prevalent in modern society. Both epidemiologic and experimental animal studies demonstrate that chronic psychological stress exerts adverse effects on the initiation and/or progression of many diseases. Here, using a mouse model of restraint stress, we report the discovery of a novel signaling pathway linking paternal stress exposure to the reprogram of hepatic gluconeogenesis in the offspring. Offspring fathered by stressed males (Stress-F1) exhibits hyperglycemia as a result of enhanced hepatic gluconeogenesis, conpared to offspring from control fathers (Control-F1). Mechanistically, protein levels of PEPCK, a key gluconeogenic enzyme, were significantly increased, while its mRNA levels were unaffected in the stress-F1 mice, pointing to a posttranscriptional regulatory mechanism. Because MicroRNAs often play an important role in regulating gene expression at the posttranscriptional level, we investigated the expression profile of MicroRNAs in livers from Control-F1 and Stress-F1 mice.
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| Overall design |
Liver tissues were collected from 12-week-old male control-F1 and stress-F1 mice (n=3 for each group). After having passed RNA quantity measurement using the NanoDrop 1000, the samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.18.0). Following the washing steps the slides were scanned using the Axon GenePix 4000B microarray scanner.
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| Contributor(s) |
Lu Y, Wu L |
| Citation missing |
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| |
| Submission date |
Sep 28, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Yan Lu |
| E-mail(s) |
lu.yan2@zs-hospital.sh.cn
|
| Phone |
+86-21-64041990
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| Organization name |
Zhongshan Hospital, Fudan University
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| Department |
Department of Endocrinology and Metabolism
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| Lab |
Zhongshan
|
| Street address |
180 Fenglin Road
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL19128 |
Exiqon miRCURY LNA microRNA array; 7th generation - hsa, mmu & rno (miRBase 18.0) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA297227 |
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