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| Status |
Public on Oct 15, 2015 |
| Title |
piRNA expression in rat ovary |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Purpose: To study the expression profile of piRNAs in rat ovary
Methods: Ovary from eight-weeks old, adult wistar rats were harvested after sacrificing the animal. The total RNA is isolated using trizol reagent. Subsequently, small RNA library was contructed using illumina kit as per manufacturer's instructions.
Results: The reads were annotated to the rat genome (rn5) and piRNA database and their expression is studied
Conclusions: Large number of piRNAs are expressed in rat ovary
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| |
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| Overall design |
Small RNA profile of rat ovary was generated by deep sequencing using Illumina HiSeq 1000
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| |
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| Contributor(s) |
Pandi G, Velmurugan G |
| Citation missing |
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| |
| Submission date |
Oct 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gopal Pandi |
| E-mail(s) |
pgp79@yahoo.com
|
| Organization name |
Madurai Kamaraj University
|
| Street address |
Department of Plant Biotechnology, School of Biotechnology
|
| City |
Madurai |
| State/province |
Tamil Nadu |
| ZIP/Postal code |
625021 |
| Country |
India |
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| Platforms (1) |
| GPL18469 |
Illumina HiSeq 1000 (Rattus norvegicus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA297891 |
| SRA |
SRP064528 |
