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Series GSE74298 Query DataSets for GSE74298
Status Public on Apr 25, 2017
Title C. neoformans gene expression by SIR2 deletion in strains H99 and RC2 subjected to glucose starvation
Organism Cryptococcus neoformans
Experiment type Expression profiling by high throughput sequencing
Summary Analysis of raw data demonstrated regulation of genes involved in transmembrane transport, transcription and translation; molecular functions that included transporter activity; and cellular components that included the cell membrane, ribosome, nucleolus, and mitochondria
 
Overall design Cryptococcus neoformans strains H99 and RC2 and respective SIR2 mutant were grown in rich media (2% glucose) or calorie restricted media (0.05% glucose) for 16h and total RNA was isolated for RNAseq
 
Contributor(s) Bouklas T, Fries BC
Citation(s) 28194146, 29463010
Submission date Oct 23, 2015
Last update date May 22, 2019
Contact name Jinsheng Yu
E-mail(s) jyu@wustl.edu
Organization name Washington University School of Medicine
Department Genetics
Lab GTAC Lab
Street address 660 S. Euclid Ave.
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL19081 Illumina HiSeq 2500 (Cryptococcus neoformans)
Samples (24)
GSM1916597 RC2-mutant grown in 2%YPD replicate 1
GSM1916598 H99-sir2mutant grown in 2% YPD replicate 1
GSM1916599 H99-wt grown in 0.05%YPD replicate 1
Relations
BioProject PRJNA299642
SRA SRP065179

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74298_RAW.tar 10.2 Mb (http)(custom) TAR (of TXT)
GSE74298_gene_counts_all.txt.gz 292.9 Kb (ftp)(http) TXT
GSE74298_transcript_counts_all.txt.gz 576.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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