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Series GSE74467 Query DataSets for GSE74467
Status Public on Oct 28, 2016
Title Snai2 and Snai3 transcriptionally regulate cellular fitness and functionality of T cell lineages through distinct gene programs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary T lymphocytes are essential contributors to the adaptive immune system and consist of multiple lineages that serve various effector and regulatory roles. As such, precise control of gene expression is essential to the proper development and function of these cells. Previously, we identified Snai2 and Snai3 as being essential regulators of immune tolerance partly due to the impaired function of CD4+ regulatory T cells in Snai2/3 conditional double knockout mice. Here we extend those previous findings using a bone marrow transplantation model to provide an environmentally unbiased view of the molecular changes imparted onto various T lymphocyte populations once Snai2 and Snai3 are deleted. The data presented here demonstrate that Snai2 and Snai3 transcriptionally regulate the cellular fitness and functionality of not only CD4+ regulatory T cells but effector CD8α+ and CD4+ conventional T cells as well. This is achieved through the modulation of gene sets unique to each cell type and includes transcriptional targets relevant to the survival and function of each T cell lineage. As such, Snai2 and Snai3 are essential regulators of T cell immunobiology.
 
Overall design GFP- CD3e+ CD8a+ CD4-, GFP- CD3e+ CD8a- CD4+ CD25- and GFP- CD3e+ CD8a- CD4+ CD25+ T cells were isolated from spleens of UBC-GFP mice transplanted with WT or cDKO lineage-depleted donor bone marrow following lethal irradiation of recipient mice. RNA-seq was performed on 3-4 biological replicates from each genotype for all T cell populations analyzed.
 
Contributor(s) Pioli PD, Whiteside SK, Weis JJ, Weis JH
Citation(s) 26831822
Submission date Oct 29, 2015
Last update date May 15, 2019
Contact name Peter Dion Pioli
E-mail(s) peter.pioli@usask.ca
Phone 1-639-994-8478
Organization name University of Saskatchewan
Department Biochemistry, Microbiology and Immunology
Street address 107 Wiggins Rd
City Saskatoon
State/province SK
ZIP/Postal code S7N 5E5
Country Canada
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (23)
GSM1921166 WT-1_CD8 (11466x1)
GSM1921167 WT-2_CD8 (11466x2)
GSM1921168 WT-3_CD8 (11466x3)
Relations
BioProject PRJNA300519
SRA SRP065478

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74467_cDKO_CD8_vs_WT_CD8.fpkm.txt.gz 749.7 Kb (ftp)(http) TXT
GSE74467_cDKO_Tcov_vs_WT_Tcov.fpkm.txt.gz 752.6 Kb (ftp)(http) TXT
GSE74467_cDKO_Treg_vs_WT_Treg.fpkm.txt.gz 701.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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