|
| Status |
Public on Oct 30, 2015 |
| Title |
Effects of Pseudorabies Virus Infection on the Tracheobronchial Lymph Node Transcriptome |
| Organism |
Sus scrofa domesticus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA isolated from draining tracheobronchial lymph nodes (TBLN) from 5-week old pigs, either clinically infected with a feral isolate of Pseudorabies virus or uninfected were interrogated using Illumina Digital Gene Expression Tag Profiling. Over 100 million tag sequences were observed, representing 4,064,189 unique 21-base sequences collected from TBLN at time points 1, 3, 6 and 14 days post-infection (dpi)
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| |
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| Overall design |
RNA isolated from draining tracheobronchial lymph nodes (TBLN) from 5-week old pigs (% per group pooled), either clinically infected with feral isolate FS268 of Pseudorabies virus or uninfected at 1, 3, 6, and 14 days post inoculation. Over 100 million tag sequences were observed, representing 4,064,189 unique 21-base sequences.
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| |
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| Contributor(s) |
Miller LC, Bayles DO, Zanella EL, Lager KM |
| Citation(s) |
26823651, 27766207 |
| |
| Submission date |
Oct 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Laura Miller |
| E-mail(s) |
laura.miller@ars.usda.gov
|
| Organization name |
USDA-ARS-National Animal Disease Center
|
| Department |
Virus and Prion Research Unit
|
| Lab |
Building 20 Room 2819 Mail Stop 2S-209
|
| Street address |
1920 Dayton Ave
|
| City |
Ames |
| State/province |
Iowa |
| ZIP/Postal code |
50010 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19790 |
Illumina HiSeq 2000 (Sus scrofa domesticus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA300530 |
| SRA |
SRP065485 |
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