NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE74493 Query DataSets for GSE74493
Status Public on Dec 11, 2015
Title Downregulation of LATS kinases alters p53 to promote cell migration
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary p53 is a pivotal tumor suppressor and a major barrier against cancer. We now report that silencing of the Hippo pathway tumor suppressors LATS1 and LATS2 in non-transformed mammary epithelial cells reduces p53 phosphorylation and increases its association with the p52 NF-?B subunit. Moreover, it partly shifts p53’s conformation and transcriptional output towards a state resembling cancer-associated p53 mutants, and endow p53 with the ability to promote cell migration. Notably, LATS1 and LATS2 are frequently downregulated in breast cancer; we propose that such downregulation might benefit cancer by converting p53 from a tumor suppressor into a tumor facilitator.
 
Overall design MCF10A cells transfected with siRNA against LATS1/2 alone, p53 alone or LATS1/2 and p53 together. Two independent MCF10A batches provided biological replicates
 
Contributor(s) Furth N, Ben-Moshe NB, Pozniak Y, Porat Z, Geiger T, Domany E, Aylon Y, Oren M
Citation(s) 26588988
Submission date Oct 29, 2015
Last update date May 15, 2019
Contact name Noa Bossel Ben-Moshe
Organization name Weizmann
Street address Hertzl Street
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (8)
GSM1921811 siControl-1
GSM1921812 siControl-2
GSM1921813 siLATS1/2-1
Relations
BioProject PRJNA300555
SRA SRP065500

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74493_RAW.tar 370.0 Kb (http)(custom) TAR (of TXT)
GSE74493_data_matrix.txt.gz 466.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.