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Status |
Public on Dec 11, 2015 |
Title |
Downregulation of LATS kinases alters p53 to promote cell migration |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
p53 is a pivotal tumor suppressor and a major barrier against cancer. We now report that silencing of the Hippo pathway tumor suppressors LATS1 and LATS2 in non-transformed mammary epithelial cells reduces p53 phosphorylation and increases its association with the p52 NF-?B subunit. Moreover, it partly shifts p53’s conformation and transcriptional output towards a state resembling cancer-associated p53 mutants, and endow p53 with the ability to promote cell migration. Notably, LATS1 and LATS2 are frequently downregulated in breast cancer; we propose that such downregulation might benefit cancer by converting p53 from a tumor suppressor into a tumor facilitator.
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Overall design |
MCF10A cells transfected with siRNA against LATS1/2 alone, p53 alone or LATS1/2 and p53 together. Two independent MCF10A batches provided biological replicates
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Contributor(s) |
Furth N, Ben-Moshe NB, Pozniak Y, Porat Z, Geiger T, Domany E, Aylon Y, Oren M |
Citation(s) |
26588988 |
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Submission date |
Oct 29, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Noa Bossel Ben-Moshe |
Organization name |
Weizmann
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Street address |
Hertzl Street
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City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (8)
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Relations |
BioProject |
PRJNA300555 |
SRA |
SRP065500 |