|
| Status |
Public on Jan 01, 2016 |
| Title |
ETO Family Protein Mtgr1 Mediates Prdm14 Functions in Stem Cell Maintenance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Prdm14 is a sequence-specific transcriptional regulator of embryonic stem cell (ESC) pluripotency and primordial germ cell (PGC) formation. It exerts its function, at least in part, through repressing genes associated with epigenetic modification and cell differentiation. Here, we show that this repressive function is mediated through an ETO-family co-repressor Mtgr1, which tightly binds to the pre-SET/SET domains of Prdm14 and co-occupies its genomic targets in mouse ESCs. Structure-guided point mutants abrogated the Prdm14-Mtgr1 association and disrupted Prdm14's function in mESC gene expression and PGC formation in vitro. Altogether, our work uncovers the molecular mechanism underlying Prdm14-mediated repression.
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| |
|
| Overall design |
Examination of Prdm14 and Mtgr1 occupancy by ChIP-seq and effects on gene expression in mouse embryonic stem cells
|
| |
|
| Contributor(s) |
Nady N, Swigut T, Wysocka J |
| Citation(s) |
26523391 |
| |
| Submission date |
Oct 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Natalie Nady |
| Organization name |
Stanford
|
| Department |
Chemical and Systems Biology
|
| Lab |
Wysocka
|
| Street address |
265 Campus Dr.
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94303 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (26)
|
|
| Relations |
| BioProject |
PRJNA300681 |
| SRA |
SRP065555 |
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