|
| Status |
Public on Nov 04, 2015 |
| Title |
Comprehensive Identification of Long Non-coding RNAs in Purified Cell Types from the Brain Reveals Functional LncRNA in OPC Fate Determination (RNA-Seq of differentiated NSC after lnc-OPC knockdown) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To quantitative analysis of transcriptome changes caused by lnc-OPC knockdown during OPC differentiation from NSC, lentivirus-based short hairpin RNAs were used to knockdown the lnc-OPC expression in a neural stem cell culture . Subsequently, puromycin-selected NSCs were differentiated to OPC in culture for three days.RNA-Seq was performed on the polyadenylated fraction of RNA isolated from cell samples. DEseq was used for differential gene expression analysis caused by lnc-OPC knockdown. GO functional term enrichment analysis of differential gene expression caused by lnc-OPC knockdown, revealed significant enrichment of ‘oligodendrocyte development’, ‘oligodendrocyte differentiation’, ‘glia cell development’, and ‘axon ensheathment’ terms that are associated with oligodendrogenesis.
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| |
|
| Overall design |
mRNA profiles of differentiiated NSC samples after lnc-OPC knockdown by RNA-sequencing.
|
| |
|
| Contributor(s) |
Dong X, Chen K, Cuevas-Diaz Duran R, Wu JQ |
| Citation(s) |
26683846 |
| |
| Submission date |
Nov 03, 2015 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Xiaomin Dong |
| E-mail(s) |
xiaomin.dong@yahoo.com
|
| Organization name |
UT health
|
| Department |
Department of neurosurgery
|
| Lab |
Dr.Jiaqian Wu
|
| Street address |
1825 Pressler Street
|
| City |
houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (4)
|
| GSM1924871 |
knockdown control (shRNA against luciferase) repeat1 |
| GSM1924872 |
knockdown control (shRNA against luciferase) repeat2 |
| GSM1924873 |
lnc-OPC knockdown repeat1 |
|
| This SubSeries is part of SuperSeries: |
| GSE74648 |
Comprehensive Identification of Long Non-coding RNAs in Purified Cell Types from the Brain Reveals Functional LncRNA in OPC Fate Determination |
|
| Relations |
| BioProject |
PRJNA301058 |
| SRA |
SRP065742 |
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