|
| Status |
Public on Jan 05, 2016 |
| Title |
A conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing [sRNA-seq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Co-transcriptional RNA processing and surveillance factors mediate heterochromatin formation in fission yeast. In addition to RNAi, RNA elimination machinery including MTREC (Mtl1-Red1 core) and the exosome are involved in facultative heterochromatin assembly, however, the exact mechanisms remain unclear. Here we show that RNA elimination factors cooperate with the conserved exoribonuclease Dhp1/Rat1/Xrn2, which couples pre-mRNA 3’-end processing to transcription termination, to promote premature termination and facultative heterochromatin formation at meiotic genes. Dhp1 also affects termination of transcripts at genes that are targets of RNAi-mediated heterochromatin assembly. Moreover, Dhp1 facilitates constitutive heterochromatin formation and silencing at centromeric and mating-type loci. Remarkably, we find that Dhp1 interacts with the Clr4/Suv39h methyltransferase complex and acts directly to nucleate heterochromatin. Our results uncover a novel role for 3’-end processing and termination machinery in gene silencing through premature termination and suggest that non-canonical termination by Dhp1 and RNA elimination factors is linked to heterochromatin assembly. These findings have important implications for understanding mechanisms of gene silencing in higher eukaryotes.
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| |
|
| Overall design |
Sequencing and analysis of small RNA in two S. pombe mutants
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| |
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| Contributor(s) |
Chalamcharla VR, Diego Folco H, Dhakshnamoorthy J, Grewal SI |
| Citation(s) |
26631744 |
| |
| Submission date |
Nov 05, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Shiv Grewal |
| Phone |
2407607553
|
| Organization name |
NCI
|
| Department |
LBMB
|
| Lab |
Shiv Grewal
|
| Street address |
NCI bldg 37 Rm 6068 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16192 |
Illumina MiSeq (Schizosaccharomyces pombe) |
|
| Samples (2) |
| GSM1931602 |
small RNA sequencing in ∆rrp6 cells |
| GSM1931603 |
small RNA sequencing in ∆rrp6 dhp1-2 cells |
|
| This SubSeries is part of SuperSeries: |
| GSE74741 |
A conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing |
|
| Relations |
| BioProject |
PRJNA301367 |
| SRA |
SRP065867 |
