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Series GSE74745 Query DataSets for GSE74745
Status Public on Nov 06, 2015
Title Expression data from brown planthhoper (BPH) infested and non-infested rice
Organism Oryza sativa
Experiment type Expression profiling by array
Summary KMD is genetically engenered to be highly resistant to lepidopteran pests through expressing a synthetic cry1Ab gene and its parent non-transgenic rice is Xiushui 11.The developmental duration of BPH feeding on KMD2 was significantly delayed. And moreover, the fecundity of BPH was significantly lower when fed on Bt rice than on the non-Bt parental plants.To investigate unintended effects in KMD2 that causes changes in BPH performance, we performed microarray (GeneChip) analysis to compare the gene expression profiles between Bt rice and non-transgenic parental plants in response to BPH infestation.
We used microarrays to detect Bt-independent variation, which might render Bt rice more defensive or less nutritious to BPH.
 
Overall design For BPH treatment, 10 second-instar nymphs were infested onto each 30-day-old seedling. After 72 h, the BPH nymphs were carefully removed and rice shoots of both BPH-infested and non-infested plants were sampled for microarray analysis. There were four treatments: Xiushui 11-non infested, Xiushui 11-BPH infested, KMD2-non infested, KMD2-BPH infested, three biological replications.
 
Contributor(s) Ye G, Wang F
Citation(s)
  • Wang F, Ning D, Chen Y, Dang C et al. Comparing Gene Expression Profiles Between Bt and non-Bt Rice in Response to Brown Planthopper Infestation. Front Plant Sci 2015;6:1181. PMID: 26734057
Submission date Nov 06, 2015
Last update date Dec 27, 2017
Contact name Gongyin Ye
E-mail(s) chu@zju.edu.cn
Organization name Zhejiang University
Street address Yuhangtang Road 866
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (12)
GSM1931628 non-infested Xiushui 11 shoots, biological rep 1
GSM1931629 non-infested Xiushui 11 shoots, biological rep 2
GSM1931630 non-infested Xiushui 11 shoots, biological rep 3
Relations
BioProject PRJNA301401

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74745_RAW.tar 50.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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