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| Status |
Public on Oct 24, 2016 |
| Title |
Analysis of Fusarium verticillioides gene expression in response to 2-benzoxazolinone (BOA) |
| Organism |
Fusarium verticillioides |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression level changes in F. verticillioides FRC M-3125 when exposed to 50 ug/ml 2-benzoxazolinone (BOA). Cultures were harvested two hours after exposure. Assessed in reference to control cultures of M-3125 exposed to ethanol (1% final concentration) since BOA was dissolved in ethanol.
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| Overall design |
A four chip study using total RNA recovered from two separate control cultures of F. verticillioides FRC M-3125 in PDB containing 1% ethanol and two separate experimental cultures of M-3125 grown in PDB plus the added treatment of 50 ug/ml ethanolic BOA. The cultures were incubated for two hours on a rotary shaker (200 rpm) at 27C in the dark and then harvested by vacuum filtration and frozen in liquid nitrogen. The chips were a F. verticillioides custom 14K array based on platform GPL14669.
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| Contributor(s) |
Glenn AE |
| Citation(s) |
26808652 |
| |
| Submission date |
Nov 09, 2015 |
| Last update date |
Oct 26, 2016 |
| Contact name |
Anthony E. Glenn |
| E-mail(s) |
anthony.glenn@usda.gov
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| Phone |
706-546-3119
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| Organization name |
USDA, ARS, USNPRC, R.B. Russell Research Center
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| Department |
Toxicology & Mycotoxin Research Unit
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| Street address |
950 College Station Rd
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30605 |
| Country |
USA |
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| Platforms (1) |
| GPL14669 |
NimbleGen F verticillioides custom 14K array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA301620 |
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