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| Status |
Public on Jun 01, 2016 |
| Title |
Next generation sequencing of Coccinella septempunctata L. with non-diapause, diapause, and diapause-terminated states |
| Organism |
Coccinella septempunctata |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The most common ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing.
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| Overall design |
The experimental insects consisted of three different states as follows: Non-diapause female insects were reared at 24±1°C, with a RH of 70±10% and a 16:8 h light: dark (L: D) photoperiod and collected after their first oviposition. Female adults in diapause were reared at 18±1°C at an RH of 70±10% and a 10:14-h (L:D) photoperiod. The experimental diapause insects were collected after 30 days. Diapause-terminated adults were transferred to another climatic cabinet with the 30-day diapause insects and reared under the same conditions as the non-diapause insects. After their first oviposition, the female insects were collected and stored at -80°C. Three biological replicates per treatment (non-diapause, diapause, diapause-terminated) were sequenced using Illumina HiSeq 2500.
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| Contributor(s) |
Qi X, Zhang L |
| Citation(s) |
26689283 |
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| Submission date |
Dec 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaoyang Qi |
| E-mail(s) |
qixiaoyang1024@163.com
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Street address |
haidian area,zhongguancun south street
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| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL21201 |
Illumina HiSeq 2500 (Coccinella septempunctata) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA304960 |
| SRA |
SRP066954 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75645_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE75645_assembly_sequence.fa.gz |
23.0 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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