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Status |
Public on May 31, 2010 |
Title |
Toxicogenomic Effect of Burkholderia pseudomallei in a Human Macrophage Cell (THP-1) Model of Acute Melioidosis Using Whole-genome Microarray |
Platform organism |
Homo sapiens |
Sample organisms |
Homo sapiens; Burkholderia pseudomallei |
Experiment type |
Expression profiling by array
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Summary |
Gene expression profiles of human cell (THP-1) lines exposed to a novel Daboiatoxin (DbTx) isolated from Daboia russelli russelli, and specific cytokines and inflammatory pathways involved in acute infection caused by Burkholderia pseudomallei. Keywords: Melioidosis, Burkholderia pseudomallei, Daboiatoxin, Cytokines, Inflammation.
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Overall design |
1. Group I:- Human monocytic macrophage (THP-1) cell lines grown in the culture medium without any bacterial infection served as untreated control group (Three Biological Replicates). 2. Group II:- THP-1 cells were infected with Burkholderia pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) for 24h served as a disease control group (Three Biological Replicates). 3. Group III:- THP-1 cells were infected with B. pseudomallei and treated with Daboiatoxin (0.5 mM) isolated from Daboia russelli russelli venom served as a treatment group (Three Biological Replicates). 4. Group IV:- THP-1 cells were infected with B. pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) treated with standard antimicrobial drug ceftazidime (10mg/ml) served as a drug control (Three Biological Replicates). 5. Group V:- THP-1 cells were exposed to Daboiatoxin (0.5 mM) without bacterial infection (Three Biological Replicates).
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Contributor(s) |
Arjunan P, Ramar PS, Ponnampalam G, Lay TA, Ooi EE |
Citation(s) |
26592245 |
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Submission date |
Apr 23, 2007 |
Last update date |
Aug 10, 2018 |
Contact name |
Pachiappan Arjunan |
Organization name |
NUS, NIH, GRU
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Department |
Anatomy, Unit on Retinal Vascular Neurobiology, BMB
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Lab |
VTRP, URVN, BMB
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Street address |
1120 15th ST, Laney walker Blvd,
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City |
Augusta |
State/province |
Georgia |
ZIP/Postal code |
30904 |
Country |
USA |
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Platforms (1) |
GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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Samples (15)
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GSM183520 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 1) |
GSM183521 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 2) |
GSM183522 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 3) |
GSM183523 |
G-(II)_THP-1+B. pseudomallei 24h_Rep1. |
GSM183525 |
G-(II)_THP-1+B. pseudomallei 24h_Rep2. |
GSM183527 |
G-(II)_THP-1+B. pseudomallei 24h_Rep3. |
GSM183530 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep1. |
GSM183532 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep2. |
GSM183533 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep3. |
GSM183534 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep1. |
GSM183535 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep2. |
GSM183536 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep3. |
GSM183537 |
G-V_THP-1+DbTx_Rep1. |
GSM183538 |
G-V_THP-1+DbTx_Rep2. |
GSM183539 |
THP-1+ treated with DbTx_Rep3. |
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Relations |
BioProject |
PRJNA100355 |
Supplementary file |
Size |
Download |
File type/resource |
GSE7577_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data provided as supplementary file |
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