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Series GSE7577 Query DataSets for GSE7577
Status Public on May 31, 2010
Title Toxicogenomic Effect of Burkholderia pseudomallei in a Human Macrophage Cell (THP-1) Model of Acute Melioidosis Using Whole-genome Microarray
Platform organism Homo sapiens
Sample organisms Homo sapiens; Burkholderia pseudomallei
Experiment type Expression profiling by array
Summary Gene expression profiles of human cell (THP-1) lines exposed to a novel Daboiatoxin (DbTx) isolated from Daboia russelli russelli, and specific cytokines and inflammatory pathways involved in acute infection caused by Burkholderia pseudomallei.
Keywords: Melioidosis, Burkholderia pseudomallei, Daboiatoxin, Cytokines, Inflammation.
 
Overall design 1. Group I:- Human monocytic macrophage (THP-1) cell lines grown in the culture medium without any bacterial infection served as untreated control group (Three Biological Replicates).
2. Group II:- THP-1 cells were infected with Burkholderia pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) for 24h served as a disease control group (Three Biological Replicates).
3. Group III:- THP-1 cells were infected with B. pseudomallei and treated with Daboiatoxin (0.5 mM) isolated from Daboia russelli russelli venom served as a treatment group (Three Biological Replicates).
4. Group IV:- THP-1 cells were infected with B. pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) treated with standard antimicrobial drug ceftazidime (10mg/ml) served as a drug control (Three Biological Replicates).
5. Group V:- THP-1 cells were exposed to Daboiatoxin (0.5 mM) without bacterial infection (Three Biological Replicates).
 
Contributor(s) Arjunan P, Ramar PS, Ponnampalam G, Lay TA, Ooi EE
Citation(s) 26592245
Submission date Apr 23, 2007
Last update date Aug 10, 2018
Contact name Pachiappan Arjunan
Organization name NUS, NIH, GRU
Department Anatomy, Unit on Retinal Vascular Neurobiology, BMB
Lab VTRP, URVN, BMB
Street address 1120 15th ST, Laney walker Blvd,
City Augusta
State/province Georgia
ZIP/Postal code 30904
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (15)
GSM183520 Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 1)
GSM183521 Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 2)
GSM183522 Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 3)
Relations
BioProject PRJNA100355

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7577_RAW.tar 43.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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