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Series GSE76073 Query DataSets for GSE76073
Status Public on Dec 05, 2016
Title Extensive regulation of diurnal transcription and metabolism by glucocorticoids [RNA-Seq]
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Altered daily patterns of hormone action are suspected to contribute to metabolic disease. It is poorly understood how the adrenal glucocorticoid hormones contribute to the coordination of daily global patterns of transcription and metabolism. Here, we examined diurnal metabolite and transcriptome patterns in a zebrafish glucocorticoid deficiency model by RNA-Seq, NMR spectroscopy and liquid chromatography-based methods. We observed dysregulation of metabolic pathways including glutaminolysis, the citrate and urea cycles and glyoxylate detoxification. Constant, non-rhythmic glucocorticoid treatment rescued many of these changes, with some notable exceptions among the amino acid related pathways. Surprisingly, the non-rhythmic glucocorticoid treatment rescued almost half of the entire dysregulated diurnal transcriptome patterns. A combination of E-box and glucocorticoid response elements is enriched in the rescued genes. This simple enhancer element combination is sufficient to drive rhythmic circadian reporter gene expression under non-rhythmic glucocorticoid exposure, revealing a permissive function for the hormones in glucocorticoid-dependent circadian transcription. Our work highlights metabolic pathways potentially contributing to morbidity in patients with glucocorticoid deficiency, even under glucocorticoid replacement therapy. Moreover, we provide mechanistic insight into the interaction between the circadian clock and glucocorticoids in the transcriptional regulation of metabolism.
 
Overall design RNA-Seq from total RNA of zebrafish larvae during (5 dpf) the diurnal cycle. Time-series mRNA profiles of untreated wild type (WT), rx3t25327/t25327 [rx3 strong] and rx3t25181/t25181 [rx3 weak] mutant larvae as well as dexamethasone treated WT and rx strong larvae were generated by deep sequencing.
 
Contributor(s) Weger BD, Weger M, Görling B, Schink A, Gobet C, Keime C, Poschet G, Jost B, Krone N, Hell R, Gachon F, Luy B, Dickmeis T
Citation(s)
  • Weger BD, Weger M, Görling B, Schink A et al. Extensive Regulation of Diurnal Transcription and Metabolism by Glucocorticoids. PLoS Genet 2016 Dec;12(12):e1006512. PMID: 27941970
Submission date Dec 16, 2015
Last update date May 15, 2019
Contact name Thomas Dickmeis
E-mail(s) thomas.dickmeis@kit.edu
Phone 004972160826564
Organization name KIT
Department ITG
Street address Hermann-von-Helmholtz-Platz 1
City Eggenstein-Leopoldshafen
ZIP/Postal code 76344
Country Germany
 
Platforms (2)
GPL15583 Illumina Genome Analyzer IIx (Danio rerio)
GPL16509 Illumina HiSeq 1000 (Danio rerio)
Samples (20)
GSM1973695 B01_wild-type_ZT03
GSM1973696 B02_wild-type_ZT09
GSM1973697 B03_wild-type_ZT15
Relations
BioProject PRJNA306153
SRA SRP067442

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76073_Normalized_counts.txt.gz 2.4 Mb (ftp)(http) TXT
GSE76073_Raw_counts.txt.gz 1012.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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