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| Status |
Public on May 31, 2006 |
| Title |
Halobacterium NRC-1, ZnSO4 Stress Response series 2 |
| Organism |
Halobacterium salinarum NRC-1 |
| Experiment type |
Expression profiling by array
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| Summary |
Given that transition metals are essential cofactors in central biological processes, misallocation of the wrong metal ion to a metalloprotein can have resounding and often detrimental effects on diverse aspects of cellular physiology. Therefore, in an attempt to characterize unique and shared responses to chemically similar metals we have reconstructed physiological behaviors of Halobacterium NRC-1, an archaeal halophile, in sub-lethal levels of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II). Over 20% of all genes responded transiently within minutes of exposure to Fe(II), perhaps reflecting immediate large scale physiological adjustments to maintain homeostasis. At steady state, each transition metal induced growth arrest, attempts to minimize oxidative stress, toxic ion scavenging, increased protein turnover and DNA repair, and modulation of active ion transport. While several of these constitute generalized stress responses, up regulation of active efflux of Co(II), Ni(II), Cu(II), and Zn(II), down regulation of Mn(II) uptake and up regulation of Fe(II) chelation, confer resistance to the respective metals. We have synthesized all these discoveries into a unified systems level model to provide an integrated perspective of responses to six transition metals with emphasis on experimentally verified regulatory mechanisms. Finally, through comparisons across global transcriptional responses to different metals we provide insights into putative in vivo metal selectivity of metalloregulatory proteins and demonstrate that a systems approach can help rapidly unravel novel metabolic potential and regulatory programs of poorly studied organisms.
Keywords: stress response, dose response
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| Overall design |
4 samples were analyzed (0mM, 0.005, 0.01 and 0.015mM Zn). Each sample was dye-swapped (2 replicates per condition) and hybridized against a standard control.
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| Citation(s) |
18160043, 16751342 |
| Submission date |
Apr 24, 2007 |
| Last update date |
Jan 15, 2017 |
| Contact name |
Marc Facciotti |
| E-mail(s) |
mfacciotti@systemsbiology.org
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| Organization name |
Institute for Systems Biology
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| Street address |
1441 North 34th Street
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
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| Platforms (1) |
| GPL3739 |
ISB Halobacterium Spotted Microarray |
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| Samples (8)
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| GSM109399 |
Control, Array 08394 |
| GSM109400 |
Control, Array 08395 |
| GSM182557 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8368 |
| GSM182558 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8369 |
| GSM182559 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8372 |
| GSM182560 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8373 |
| GSM182561 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8374 |
| GSM182562 |
Halobacterium Zinc Sulfate stress exposure experiment, Array 8375 |
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| This SubSeries is part of SuperSeries: |
| GSE4925 |
Halobacterium NRC-1 response to sub-lethal levels of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) |
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| Relations |
| BioProject |
PRJNA100177 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7611_RAW.tar |
7.0 Mb |
(http)(custom) |
TAR (of CSV) |
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