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Series GSE76110 Query DataSets for GSE76110
Status Public on Apr 06, 2016
Title Transcriptomics Responses to Hypoxia and Ketamine in Ovine Fetal Cerebral Cortex
Organism Ovis aries
Experiment type Expression profiling by array
Summary Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. The chemoreceptor reflex that is responsible for this physiological response is dependent on glutamatergic neurotransmission which, in times of vigorous activity, could produce cell death secondary to calcium uptake. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist of NMDA receptors, reduces the damage. Late-gestation, chronically catheterized fetal sheep were subjected to a 30 min period of ventilatory hypoxia that decreased fetal PaO2 from 17±1 to 10±1 mm Hg, or normoxia (PaO2 17±1 mm Hg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, iv). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed and mRNA extracted for transcriptomics and systems biology analysis. Hypoxia stimulated a transcriptomics response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic system biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response.
 
Overall design 4 groups: hypoxia, hypoxia plus ketamine, normoxia, normoxia plus ketamine. Hypoxia produced by low PO2 in maternal inspired gas for 30 min, followed by normoxia recovery for 23.5 hours. Control fetuses maintained at normoxia for 30 min, followed by another 23.5 h of normoxia. Fetal frontal cerebral cortex collected for mRNA at end of 23.5 h recovery period.
 
Contributor(s) Chang EI, Zarate M, Rabaglino MB, Richards EM, Arndt TJ, Keller-Wood M, Wood CE
Citation(s) 27033443
Submission date Dec 17, 2015
Last update date Apr 06, 2016
Contact name Charles Evans Wood
E-mail(s) woodc@ufl.edu
Phone 352-294-5064
Organization name University of Florida
Department Physiology and Functional Genomics
Street address 1345 Center Drive/Room M552
City GAINESVILLE
State/province Florida
ZIP/Postal code 32610-0274
Country USA
 
Platforms (1)
GPL10427 Agilent-019921 Sheep Gene Expression Microarray (Feature Number version)
Samples (16)
GSM1974387 normoxia control (no ketamine) replicate 1
GSM1974388 normoxia control (no ketamine) replicate 2
GSM1974389 normoxia control (no ketamine) replicate 3
Relations
BioProject PRJNA306253

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76110_RAW.tar 43.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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