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Status |
Public on May 04, 2007 |
Title |
The S. cerevisiae histone demethylase Jhd1 fine-tunes the distribution of H3K36me2 |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
Histone methylation plays important roles in the regulation of chromatin dynamics and transcription. Steady state levels of histone lysine methylation are regulated by a balance between enzymes that catalyze either the addition or removal of methyl groups. Using an activity-based biochemical approach, we recently uncovered the JmjC domain as an evolutionarily conserved signature motif for histone demethylases. Furthermore, we demonstrated that Jhd1, a JmjC domain-containing protein in S. cerevisiae, is an H3K36-specific demethylase. Here we report further characterization of Jhd1. Similar to its mammalian homolog, Jhd1-catalyzed histone demethylation requires iron and alpha-ketoglutarate as cofactors. Mutation and deletion studies indicate that the JmjC domain and adjacent sequences are critical for Jhd1 enzymatic activity, while the N-terminal PHD domain is dispensable. Overexpression of JHD1 results in a global reduction of H3K36 methylation in vivo. Finally, chromatin immunoprecipitation coupled microarray (ChIP-chip) studies reveal subtle changes in the distribution of H3K36me2 upon overexpression or deletion of JHD1. Our studies establish Jhd1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units. Keywords: ChIP-chip
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Overall design |
H3K36me2 ChIPs were performed on wild type, jhd1 knockout, and JHD1 overexpression yeast strains.
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Contributor(s) |
Fang J, Hogan GJ, Liang G, Lieb JD, Zhang Y |
Citation(s) |
17470555 |
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Submission date |
Apr 26, 2007 |
Last update date |
Jul 26, 2013 |
Contact name |
Jason D Lieb |
E-mail(s) |
jlieb@bio.unc.edu
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Phone |
919-842-3228
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Organization name |
University of North Carolina at Chapel Hill
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Department |
Department of Biology
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Lab |
Jason Lieb
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Street address |
203 Fordham Hall
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City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-3280 |
Country |
USA |
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Platforms (1) |
GPL5125 |
Lieb Lab at UNC-CH_Yeast Whole-genome and Chr III Tiling Array (PCR-based) |
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Samples (11)
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GSM184455 |
H3K36me2 ChIP in JHD1 OE strain #1 (dye swap) |
GSM184456 |
H3K36me2 ChIP in JHD1 OE strain #2 |
GSM184457 |
H3K36me2 ChIP in wild type strain #2 |
GSM184458 |
H3K36me2 ChIP in wild type strain #1 (dye swap) |
GSM184459 |
H3K36me2 ChIP in jhd1 KO strain #1 (dye swap) |
GSM184460 |
H3K36me2 ChIP in jhd1 KO strain #2 |
GSM184461 |
H3K36me2 ChIP in wild type strain #3 |
GSM184462 |
H3K36me2 ChIP in jhd1 KO strain #3 |
GSM184463 |
H3K36me2 ChIP in wild type strain #1 |
GSM184464 |
H3K36me2 ChIP in jhd1 KO strain #1 |
GSM184465 |
H3K36me2 ChIP in JHD1 OE strain #3 (dye swap) |
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Relations |
BioProject |
PRJNA100253 |