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Series GSE7627 Query DataSets for GSE7627
Status Public on May 04, 2007
Title The S. cerevisiae histone demethylase Jhd1 fine-tunes the distribution of H3K36me2
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Histone methylation plays important roles in the regulation of chromatin dynamics and transcription. Steady state levels of histone lysine methylation are regulated by a balance between enzymes that catalyze either the addition or removal of methyl groups. Using an activity-based biochemical approach, we recently uncovered the JmjC domain as an evolutionarily conserved signature motif for histone demethylases. Furthermore, we demonstrated that Jhd1, a JmjC domain-containing protein in S. cerevisiae, is an H3K36-specific demethylase. Here we report further characterization of Jhd1. Similar to its mammalian homolog, Jhd1-catalyzed histone demethylation requires iron and alpha-ketoglutarate as cofactors. Mutation and deletion studies indicate that the JmjC domain and adjacent sequences are critical for Jhd1 enzymatic activity, while the N-terminal PHD domain is dispensable. Overexpression of JHD1 results in a global reduction of H3K36 methylation in vivo. Finally, chromatin immunoprecipitation coupled microarray (ChIP-chip) studies reveal subtle changes in the distribution of H3K36me2 upon overexpression or deletion of JHD1. Our studies establish Jhd1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units.
Keywords: ChIP-chip
 
Overall design H3K36me2 ChIPs were performed on wild type, jhd1 knockout, and JHD1 overexpression yeast strains.
 
Contributor(s) Fang J, Hogan GJ, Liang G, Lieb JD, Zhang Y
Citation(s) 17470555
Submission date Apr 26, 2007
Last update date Jul 26, 2013
Contact name Jason D Lieb
E-mail(s) jlieb@bio.unc.edu
Phone 919-842-3228
Organization name University of North Carolina at Chapel Hill
Department Department of Biology
Lab Jason Lieb
Street address 203 Fordham Hall
City Chapel Hill
State/province NC
ZIP/Postal code 27599-3280
Country USA
 
Platforms (1)
GPL5125 Lieb Lab at UNC-CH_Yeast Whole-genome and Chr III Tiling Array (PCR-based)
Samples (11)
GSM184455 H3K36me2 ChIP in JHD1 OE strain #1 (dye swap)
GSM184456 H3K36me2 ChIP in JHD1 OE strain #2
GSM184457 H3K36me2 ChIP in wild type strain #2
Relations
BioProject PRJNA100253

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE7627_RAW.tar 17.9 Mb (http)(custom) TAR (of GPR)

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