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| Status |
Public on Jan 07, 2016 |
| Title |
SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing [CLIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
SIRT7 is an NAD+-dependent protein deacetylase with important roles in ribosome biogenesis and cell proliferation. Previous studies have established that SIRT7 is associated with RNA polymerase I, interacts with pre-rRNA and promotes rRNA synthesis. Here we show that SIRT7 is also associated with snoRNAs that are involved in pre-rRNA processing and rRNA maturation. Knockdown of SIRT7 impairs U3 snoRNA-dependent early cleavage steps that are necessary for generation of 18S rRNA. Mechanistically, SIRT7 deacetylates U3-55k, a core component of the U3 snoRNP complex, and reversible acetylation of U3-55k modulates the association of U3-55k with U3 snoRNA. Deacetylation by SIRT7 enhances U3-55k binding to U3 snoRNA, which is a prerequisite for pre-rRNA processing. Under stress conditions, SIRT7 is released from nucleoli, leading to hyperacetylation of U3-55k and attenuation of prerRNA processing. The results reveal a multifaceted role of SIRT7 in ribosome biogenesis, regulating both transcription and processing of rRNA.
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| Overall design |
CLIP-seq was performed in Flag-SIRT7-293T cells.
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| Contributor(s) |
Chen S, Blank MF, Iyer A, Huang B, Wang L, Grummt I, Voit R |
| Citation(s) |
26867678 |
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| Submission date |
Jan 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Renate Voit |
| E-mail(s) |
r.voit@dkfz.de
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| Organization name |
German Cancer Research Center
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| Department |
Division Molecular Biology of the Cell
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| Street address |
Im Neuenheimer Feld 581
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA308081 |
| SRA |
SRP068144 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76578_snoRNAs_identified_SIRT7_CLIP_seq.xlsx |
41.1 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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