|
| Status |
Public on Sep 16, 2016 |
| Title |
The control of rRNA synthesis during the directed differentiation of human embryonic stem cells precedes heterochromatin formation. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Third-party reanalysis
|
| Summary |
It has become increasingly clear that proper cellular control of pluripotency and differentiation is related to the regulation of rRNA synthesis. To further our understanding of the role that the regulation of rRNA synthesis has on pluripotency we monitored the reduction of rRNA synthesis after the induction of endoderm-specific gene expression in human embryonic stem cells (hESCs) using a brief Activin A treatment. We discovered that the reduction in rRNA synthesis during directed differentiation is rapid (within 6 hours of Activin A treatment), correlates with a reduction in the binding of UBTF, and precedes heterochromatin formation throughout the rRNA gene. The loss of pluripotency and the induction of lineage specific gene expression markers can be induced by reducing rRNA synthesis directly with the Pol I inhibitor, CX-5461. Activin A and CX-5461 both appear to reduce pluripotency and alter lineage-specific transcription, but likely do so via distinct mechanisms.
|
| |
|
| Overall design |
UBF Binding by ChIP-seq in untreated and Activin A treated H9 hESCS. mRNA expression level by mRNA-seq in untreated, Activin A treated, and CX-5461 treated H9 hESCs
|
| |
|
| Contributor(s) |
Giles KE, Woolnough JL |
| Citation(s) |
27299313 |
| |
| Submission date |
Jan 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jessica L Woolnough |
| E-mail(s) |
jlmakofske@gmail.com
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Genetics
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (21)
|
|
| Relations |
| Reanalysis of |
GSM1006724 |
| Reanalysis of |
GSM1006725 |
| Reanalysis of |
GSM1006726 |
| Reanalysis of |
GSM1006727 |
| BioProject |
PRJNA308103 |
| SRA |
SRP068151 |
Less...
More...