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| Status |
Public on Dec 26, 2018 |
| Title |
Overexpression of Crumbs3/CRB3 in human mammary epithelial line MCF-10A |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Our data demonstrate that overexpression of the polarity protein Crb3 elicits changes in MCF-10A cells that culminate in an increase in the release of amphiregulin (AR) and the subsequent activation of EGFR signaling to drive proliferation. Microarray analysis was performed to define global changes in the transcriptional landscape induced by Crb3. Results provide insight into a FERM domain protein (EBP41L4B) required for Crb3 mediated induction of proliferation.
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| |
|
| Overall design |
Total RNA was isolated from MCF-10A cells infected with pNG vector control or pNG-Crb3 retrovirus.
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| |
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| Contributor(s) |
Walker SJ, Selfors LM, Margolis BL, Brugge JS |
| Citation(s) |
30440051 |
| |
| Submission date |
Jan 06, 2016 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Joan S. Brugge |
| E-mail(s) |
joan_brugge@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Department of Cell Biology
|
| Lab |
Brugge
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
|
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| Relations |
| BioProject |
PRJNA308137 |
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