|
| Status |
Public on Jul 14, 2016 |
| Title |
Differential gene expression m39 vs. siblings |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNAseq analysis of cloche m39 mutant zebrafish embryos and wild type siblings at 90% epiboly - tailbud stage
|
| |
|
| Overall design |
In order to isolate the cloche gene, RNAseq was performed on a deletion allele of the zebrafish cloche mutant. RNA was extracted from individual embryos at a stage the cloche gene was predicted to be expressed based on previous literature. RNA from the respective genoptypes was then pooled and subjected to RNAseq analysis.
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| |
|
| Contributor(s) |
Stainier D, Reischauer S, Stone O, Villasenor A |
| Citation(s) |
27411634 |
| |
| Submission date |
Jan 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Miler T Lee |
| E-mail(s) |
miler@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Biological Sciences
|
| Street address |
4249 Fifth Ave.
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15260 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15583 |
Illumina Genome Analyzer IIx (Danio rerio) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA308321 |
| SRA |
SRP068242 |
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