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| Status |
Public on Jan 12, 2016 |
| Title |
The impact of transgenic Uchl1 on gene expression in germinal center B-cells from ImHABCL6 mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study seeks to understand the mechanisms behind enhanced lymphomagenesis observed in ImHABCL6/Uchl1 mice compared with ImHABCL6 alone. As the lymphomas arise from germinal center (GC) B-cells, we reasoned that transgenic Uchl1 altered the gene expression patterns in GC B-cells from these animals. We therefore isolated pre-malignant GC B-cells and examined the gene expression patterns to identify pathways affected by the addition of Uchl1.
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| Overall design |
Germinal center B-cells were isolated from from the spleens of ImHABCL6 and ImHABCL6/Uchl1 mutant mice (n= 3 each) after immunization with sheep red blood cells. RNA was extracted and hybridized to affymetrix mouse 430 2.0 arrays.
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| Contributor(s) |
Bedekovics T, Galardy PJ |
| Citation(s) |
26702068 |
| Submission date |
Jan 11, 2016 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Asha Nair |
| E-mail(s) |
nair.asha@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Street address |
200 1ST ST SW
|
| City |
Rochester |
| ZIP/Postal code |
55901 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA308434 |
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