|
| Status |
Public on May 03, 2007 |
| Title |
Effects of capture-release events in wild dolphins: PBL analysis |
| Organism |
Tursiops truncatus |
| Experiment type |
Expression profiling by array
|
| Summary |
Goal: To determine the effects of capture-release events in wild dolphins (Tursiops truncatus).
Methods: An analysis of the Peripheral Blood Leukocyte (PBL) transcriptome was conducted on a group of 20 animals. The samples were collected in 2 different locations along the US east coast (Charleston, SC; Indian River Lagoon, FL) and 2 blood samples were collected for each dolphin 1) immediately after the capture event (*pre*) and 2) just before the animal was released (*post*). In between *pre* and *post* blood collections (30-40 minutes) additional samples were collected from the animals for physiological, chemical and biochemical analysis. RNA extracted from *pre* and *post* blood samples was used for micorarray hybridizations and transcriptome analysis using a species-specific PBL cDNA microarray (Mancia *et al*., 2007).
Keywords: blood cells (PBL)
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| |
|
| Overall design |
time course, field collection/wild caught organisms, For multiple hybridizations: serial, biological replicates
|
| Web link |
http://www.marinegenomics.org/
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| |
|
| Contributor(s) |
Mancia A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 02, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
MarineGenomics Project |
| E-mail(s) |
info@marinegenomics.org
|
| Phone |
843-762-8869
|
| Fax |
843-762-8847
|
| URL |
http://www.marinegenomics.org
|
| Organization name |
MarineGenomics.org
|
| Lab |
HML
|
| Street address |
331 Fort Johnson Rd
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29412 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3609 |
Tursiops truncatus cDNA Microarray version 1.0 |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA99449 |
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