NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE76915 Query DataSets for GSE76915
Status Public on Jan 16, 2016
Title A novel role for ASCL1 in the regulation of mesendoderm formation via HDAC-dependent antagonism of VegT function
Organism Xenopus laevis
Experiment type Expression profiling by high throughput sequencing
Summary Maternally expressed proteins function in vertebrates to establish the major body axes of the embryo, and to establish a pre-pattern that sets the stage for later acting zygotic signals. This pre-pattern drives the propensity of Xenopus animal cap cells to adopt neural fates under various experimental conditions. Previous studies found that the maternally expressed transcription factor, encoded by the Xenopus achaete-scute like gene ascl1, is enriched at the animal pole. Asc1l is a bHLH protein involved in neural development, but its maternal function has not been studied. In this study, we have performed a series of gain and loss of function experiments on maternal ascl1, and present three novel findings. First, Ascl1 is a repressor of mesendoderm induced by VegT, but not of Nodal induced mesendoderm. Secondly, a previously uncharacterized N-terminal domain of Ascl1 interacts with HDAC1 to inhibit mesendoderm gene expression. This N-terminal domain is dispensable for its neurogenic function, indicating that Ascl1 has acts by different mechanisms at different times. Ascl1-mediated repression of mesendoderm genes was dependent on HDAC activity and accompanied by histone deacetylation in the promoter regions of VegT targets. Finally, maternal Ascl1 is required for animal cap cells to retain their competence to adopt neural fates. These results establish maternal Asc1l as a key factor in establishing the pre-pattern of the early embryo, acting in opposition to VegT and biasing the animal pole to adopt neural fates. The data presented here significantly extend our understanding of early embryonic pattern formation.
 
Overall design Examination of genes expression in control (cMO) and Ascl1 MO knockdown (AMOs) embryos by deep sequencing.
 
Contributor(s) Gao L, Ma X, Tao Q
Citation(s) 26700681
Submission date Jan 15, 2016
Last update date May 15, 2019
Contact name Li Gao
E-mail(s) gao-ly@163.com
Organization name Tsinghua University
Department School of Life Sciences
Lab Development Biology Lab
Street address Qinghuayuan Street
City Beijing
ZIP/Postal code 100084
Country China
 
Platforms (1)
GPL18936 Illumina HiSeq 2500 (Xenopus laevis)
Samples (6)
GSM2040681 cMO rep1
GSM2040682 cMO rep2
GSM2040683 cMO rep3
Relations
BioProject PRJNA308931
SRA SRP068506

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76915_AMOs.count.txt.gz 363.6 Kb (ftp)(http) TXT
GSE76915_cMO.count.txt.gz 370.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap