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| Status |
Public on Jul 27, 2016 |
| Title |
Functional maturation of rat beta cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The weaning period consist of a critical postnatal window for structural and physiologic maturation of rat beta cells. To investigate transcriptome changes involved in the maturation of beta cells neighboring this period we performed microarray analysis in FACS beta cell enriched populations to detail the global programme of gene expression to identify its changes during this process.
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| Overall design |
Male Wistar rats were selected at the initial point of a postnatal critical period (postnatal 20d) for pancreatic maturation for RNA extraction and hybridization on Affymetrix microarrays. We obtained immature and mature beta cell-enriched populations from postnatal 20d and 2 months animals in order to compare their expression profiles. To that end, we used primary cultured FACS-enriched beta cell populations according to their FSC, SSC and autoflourescence. We used three replicates for each condition.
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| Contributor(s) |
Larqué C, Velasco M, Barajas F, García-Delgado N, Chávez-Maldonado JP, García-Morales J, Orozco L, Hiriart M |
| Citation(s) |
27220619 |
| |
| Submission date |
Jan 19, 2016 |
| Last update date |
Jul 27, 2016 |
| Contact name |
Carlos Alfonso Larqué Velázquez |
| E-mail(s) |
skiuty@gmail.com
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| Organization name |
Instituto de Fisiología Celular UNAM
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| Department |
Neurodevelopment and Physiology
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| Lab |
BL-102
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| Street address |
Circuito Exterior s/n Ciudad Universitaria. Del. Coyoacán. Mexico City
|
| City |
Distrito Federal |
| State/province |
Distrito Federal |
| ZIP/Postal code |
04360 |
| Country |
Mexico |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2041684 |
FAC-sorted adult beta cells, biological rep1 |
| GSM2041685 |
FAC-sorted adult beta cells, biological rep2 |
| GSM2041686 |
FAC-sorted adult beta cells, biological rep3 |
| GSM2041687 |
FAC-sorted 20d beta cells, biological rep1 |
| GSM2041688 |
FAC-sorted 20d beta cells, biological rep2 |
| GSM2041689 |
FAC-sorted 20d beta cells, biological rep3 |
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| Relations |
| BioProject |
PRJNA309161 |
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