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| Status |
Public on Apr 03, 2016 |
| Title |
The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome |
| Organism |
Acropora digitifera |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite the ecological significance of the relationship between reef-building corals and intracellular photosynthetic dinoflagellates of the genus Symbiodinium, very little is known about the molecular mechanisms involved in the establishment of the relationship. Indeed, microarray-based analyses point to the conclusion that host gene expression is largely or completely unresponsive during the establishment of symbiosis with a competent strain of Symbiodinium. In the present study, the use of Illumina RNAseq technology allowed detection of a transient period of differential expression involving a small number of genes (1073 transcripts; <3% of the transcriptome) 4h after the exposure of Acropora digitifera planulae to a competent strain of Symbiodinium (a clade B strain). This phenomenon has not previously been detected as a consequence of both the lower sensitivity of the microarray approaches used and the sampling times used. The results imply that complex changes occur, including transient suppression of mitochondrial metabolism and protein synthesis, but are also consistent with the hypothesis that the symbiosome is a phagosome that has undergone early arrest, raising the possibility of common mechanisms in the symbiotic interactions of corals and symbiotic sea anemones with their endosymbionts.
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| Overall design |
There were 2 conditions (Symbiodinium-infected and control). Samples were taken at 3 time points, there were 3 replicates per condition. 16 samples were analysed comparing the Symbiodinium-infected samples to the control ones
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| Contributor(s) |
Mohamed AR, Cumbo VR, Harii S, Shinzato C, Chan CX, Ragan MA, Bourne DG, Willis BL, Ball EE, Satoh N, Miller DJ |
| Citation(s) |
27094992 |
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| Submission date |
Jan 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Amin Roushdy Mohamed |
| E-mail(s) |
am_rd85@yahoo.com
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| Organization name |
CSIRO
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| Department |
Agriculture and Food
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| Lab |
Aquaculture Genomics
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| Street address |
306 Carmody Rd, University of Queensland
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL21348 |
Illumina HiSeq 2000 (Acropora digitifera) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA309168 |
| SRA |
SRP068580 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76976_04H_matrix.TMM.fpkm.matrix.txt.gz |
620.3 Kb |
(ftp)(http) |
TXT |
| GSE76976_04H_matrix.counts.matrix.txt.gz |
444.8 Kb |
(ftp)(http) |
TXT |
| GSE76976_12H_matrix.TMM.fpkm.matrix.txt.gz |
433.6 Kb |
(ftp)(http) |
TXT |
| GSE76976_12H_matrix.counts.matrix.txt.gz |
305.7 Kb |
(ftp)(http) |
TXT |
| GSE76976_48H_matrix.TMM.fpkm.matrix.txt.gz |
572.6 Kb |
(ftp)(http) |
TXT |
| GSE76976_48H_matrix.counts.matrix.txt.gz |
374.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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