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Status |
Public on Jan 22, 2016 |
Title |
Perivascular Progenitor Cells Derived from Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes, and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Diabetic retinopathy (DR) is the leading cause of blindness in working-age people. Pericyte loss is one of the pathologic cellular events in DR, which weakens the retinal microvessels. Damages to the microvascular networks are irreversible and permanent, thus further progression of DR is inevitable. In this study, we hypothesize that multipotent perivascular progenitor cells derived from human ESCs (hESC-PVPCs) improve the damaged retinal vasculature in the streptozotocin (STZ)-induced diabetic rodent models. We describe a highly efficient and feasible protocol to derive such cells using a natural selection method without cell sorting processes. As a cellular model of pericytes, hESC-PVPCs exhibited marker expressions such as CD140B, CD146, NG2, and functional characteristics of pericytes. Following a single intravitreal injection into diabetic Brown Norway (BN) rats, we demonstrate that the cells localized alongside typical perivascular regions of the retinal vasculature, and stabilized blood-retinal barrier (BRB) breakdown. Findings in this study highlight a therapeutic potential of hESC-PVPCs in DR by mimicking the role of pericytes in vascular stabilization.
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Overall design |
The molecular profiling analysis of ontological selected gene sets demonstrated that the transcripts associated with stemness markers, neural crest, neuro-ectoderm, and endoderm gene expressions.
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Contributor(s) |
Kim J, Hong K, Song W, Bae D, Chung H |
Citation(s) |
27388242 |
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Submission date |
Jan 21, 2016 |
Last update date |
Aug 13, 2018 |
Contact name |
Hyung-Min Chung |
E-mail(s) |
stemness@gmail.com
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Organization name |
Konkuk university school of medicine
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Department |
Department of Medicine
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Lab |
Stem cell research
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Street address |
120 Neungdong-ro, Gwangjin-gu
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City |
Seoul |
ZIP/Postal code |
143-701 |
Country |
South Korea |
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Platforms (1) |
GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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Samples (9)
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GSM2044507 |
human embryoid body_BMP4 treated_6days_rep1 |
GSM2044508 |
human embryoid body_BMP4 treated_6days_rep2 |
GSM2044509 |
human embryoid body_BMP4 treated_6days_rep3 |
GSM2044510 |
human embryonic stem cell derived perivascular progenitor cell_experiment_rep1 |
GSM2044511 |
human embryonic stem cell derived perivascular progenitor cell_experiment_rep2 |
GSM2044512 |
human embryonic stem cell derived perivascular progenitor cell_experiment_rep3 |
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Relations |
BioProject |
PRJNA309455 |