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| Status |
Public on Apr 18, 2017 |
| Title |
Expression data from young and senescent HCAECs treated with proton pump inhibitors (omeprazole and lansoprazole) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Proton pump inhibitors (PPIs) are among the most frequently prescribed drugs, especially in older people. Although these drugs are usually considered safe, recent evidence suggests that high dose and/or long term use of PPIs may have several detrimental effects, including increased risk of adverse cardiovascular events. The impact of PPI in the aging host environment still need to be characterized. Aged tissues, including vascular tissues, accumulate senescent cells that can communicate with their environment by secreting a myriad of cytokines and growth factors. Human coronary artery endothelial cells (HCAECs) provide an excellent model system to study “in vitro” most aspects of cardiovascular function and disease related to cellular senescence. The purpose of this study is thus to investigate the in vitro effects of two well-known PPIs (Omeprazole and Lansoprazole) on endothelial gene expression in senescent e non-senescent HCAECs.
We used a cDNA microarray method to compare gene expression profiles of young and senescent HCAECs treated with omeprazole and lansoprazole.
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| Overall design |
Young cells were cultured in medium supplemented with vehicle (CTRL) or 100μM PPIs (Omeprazole or Lansoprazole) and grown for subsequent passages until they evidenced senescence-associated phenotypes. Total mRNA was extracted and gene expression profiles were analyzed in senescent endothelial cells (P12) compared to the non-senescent cells (P6) in both untreated (CTRL) and PPI-treated groups by cDNA microarray. All experiments were performed in triplicate for each treatment group.
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| Contributor(s) |
Costarelli L |
| Citation(s) |
28039570 |
| |
| Submission date |
Jan 26, 2016 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Laura Costarelli |
| E-mail(s) |
l.costarelli@inrca.it
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| Organization name |
INRCA - National Institute of Health & Science on Ageing
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| Street address |
Via Birarelli, 8
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| City |
Ancona |
| ZIP/Postal code |
60121 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (18)
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| GSM2046430 |
endothelial cells_old_control_rep1 |
| GSM2046431 |
endothelial cells_old_control_rep2 |
| GSM2046432 |
endothelial cells_old_control_rep3 |
| GSM2046433 |
endothelial cells_young_omeprazole100μM_rep1 |
| GSM2046434 |
endothelial cells_young_omeprazole100μM_rep2 |
| GSM2046435 |
endothelial cells_young_omeprazole100μM_rep3 |
| GSM2046436 |
endothelial cells_old_omeprazole100μM_rep1 |
| GSM2046437 |
endothelial cells_old_omeprazole100μM_rep2 |
| GSM2046438 |
endothelial cells_old_omeprazole100μM_rep3 |
| GSM2046439 |
endothelial cells_young_lansoprazole100μM_rep1 |
| GSM2046440 |
endothelial cells_young_lansoprazole100μM_rep2 |
| GSM2046441 |
endothelial cells_young_lansoprazole100μM_rep3 |
| GSM2046442 |
endothelial cells_old_lansoprazole100μM_rep1 |
| GSM2046443 |
endothelial cells_old_lansoprazole100μM_rep2 |
| GSM2046444 |
endothelial cells_old_lansoprazole100μM_rep3 |
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| Relations |
| BioProject |
PRJNA309835 |
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