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| Status |
Public on Jul 30, 2016 |
| Title |
Deep transcriptome analysis of mouse embryonic fibroblast following simultaneous overexpression of Stella, Oct4 and Nanos2 (SON) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We constructed a polycistronic lentiviral vector to overexpress 3 germ cell specific genes (Stella, Oct4 and Nanos2) in mouse embryonic fibroblast (MEFs) and evaluated the transcriptome portrait in partially reprogrammed cells.We sequenced RNA samples from bulk cell population of two biological duplicates of MEF-GFP (control) and MEF-SON (overexpressed) 21 days post infection. Differential expression analysis of 50 M pair-end read per samples showed overexpression of neurogenesis, blood vessel and proliferation related genes and downregulation of chondroitin sulphate metabolic process, nitric oxide production and innate immune response genes.
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| Overall design |
Examination of whole transcriptome following concurrent overexpression of Stella, Oct4 and Nanos2 in MEFs.
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| Contributor(s) |
Farshchian M, Armant O, Irimia M, Brattås PL, Matin MM, Bahrami AR |
| Citation(s) |
29501710 |
| |
| Submission date |
Jan 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Moein Farshchian |
| E-mail(s) |
moeinfarshchy@yahoo.com
|
| Organization name |
Ferdowsi University of Mashhad
|
| Department |
Department of Biology,Faculty of Science,
|
| Street address |
Vakilabad Highway
|
| City |
Mashhad |
| ZIP/Postal code |
9177948974 |
| Country |
Iran |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA310119 |
| SRA |
SRP069024 |
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