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| Status |
Public on Jan 30, 2016 |
| Title |
Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes.
The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268
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| Overall design |
A 12 RNA-seq study was performed. Based on GFP intensity, equal number (4-5.5 X 10 6 ) of mature male and female gametocytes were separated by cell-sorter. Total RNA with and without rRNA depletion were processed for generation libraries using the Illumina TruSeq Stranded mRNA Library Preparation kit (Illumina Inc., San Diego, CA). Libraries were sequenced on an Illumina HiSeq 2500 in Rapid Run mode using 100 nucleotide single read sequencing. Altogether, this study investigated three experimental replicates of transcriptomes for mRNA and rRNA from male and female gametocytes (12 samples)
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| Contributor(s) |
Jun M, Liwang C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
JUN MIAO |
| E-mail(s) |
jmiao1@usf.edu
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| Phone |
8139747374
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| Organization name |
University of South Florida
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| Department |
Internal Medicine
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| Lab |
Jun Miao and Liwang Cui
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| Street address |
3720 Spectrum Boulevard, MDC84
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| City |
Tampa |
| State/province |
Florida |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL21078 |
Illumina HiSeq 2500 (Plasmodium falciparum) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA310220 |
| SRA |
SRP069075 |
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