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Series GSE77398 Query DataSets for GSE77398
Status Public on Jan 30, 2016
Title Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes
Organism Plasmodium falciparum
Experiment type Expression profiling by high throughput sequencing
Summary Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes.
The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268
 
Overall design A 12 RNA-seq study was performed. Based on GFP intensity, equal number (4-5.5 X 10 6 ) of mature male and female gametocytes were separated by cell-sorter. Total RNA with and without rRNA depletion were processed for generation libraries using the Illumina TruSeq Stranded mRNA Library Preparation kit (Illumina Inc., San Diego, CA). Libraries were sequenced on an Illumina HiSeq 2500 in Rapid Run mode using 100 nucleotide single read sequencing. Altogether, this study investigated three experimental replicates of transcriptomes for mRNA and rRNA from male and female gametocytes (12 samples)
 
Contributor(s) Jun M, Liwang C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 29, 2016
Last update date May 15, 2019
Contact name JUN MIAO
E-mail(s) jmiao1@usf.edu
Phone 8139747374
Organization name University of South Florida
Department Internal Medicine
Lab Jun Miao and Liwang Cui
Street address 3720 Spectrum Boulevard, MDC84
City Tampa
State/province Florida
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL21078 Illumina HiSeq 2500 (Plasmodium falciparum)
Samples (12)
GSM2051314 Male 1-mRNA
GSM2051315 Female 1-mRNA
GSM2051316 Male 2-mRNA
Relations
BioProject PRJNA310220
SRA SRP069075

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77398_deseq-kallisto-mRNA-counts_v3.1.xls.gz 476.2 Kb (ftp)(http) XLS
GSE77398_deseq-kallisto-ribo-counts_v3.1.xls.gz 446.8 Kb (ftp)(http) XLS
GSE77398_mal_all6_cuffdiff_genes.xls.gz 567.0 Kb (ftp)(http) XLS
GSE77398_ribo_cuffdiff_genes.xls.gz 545.4 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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