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Status |
Public on Feb 01, 2017 |
Title |
Organ-specific gene expression in single differentiating Drosophila embryos |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Age-matched stage 13 embryos were dissected and cDNA of 8 organs from each embryo were sequenced. The organs were foregut, anterior midgut, posterior midgut, hindgut, Malpighian tubules, left salivary gland, right salivary gland, ventral nerve cord. Gene expression, transcription start and stop sites, and strands of termini were analysed. SMART amplification adapters were used for finding transcript termini and for phasing.
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Overall design |
3 embryos, 8 organs from each. PolyA RNA was reverse transcribed and amplified using an improved SMART protocol.
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Contributor(s) |
Molnar D |
Citation missing |
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Submission date |
Feb 01, 2016 |
Last update date |
May 15, 2019 |
Contact name |
David Molnar |
Organization name |
University of Cambridge
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Department |
Genetics / Systems Biology Centre
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Street address |
Tennis Court Road
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City |
Cambridge |
ZIP/Postal code |
CB2 1QR |
Country |
United Kingdom |
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Platforms (1) |
GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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Samples (24)
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Relations |
BioProject |
PRJNA310323 |
SRA |
SRP069131 |