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Series GSE77429 Query DataSets for GSE77429
Status Public on Feb 01, 2017
Title Organ-specific gene expression in single differentiating Drosophila embryos
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Age-matched stage 13 embryos were dissected and cDNA of 8 organs from each embryo were sequenced. The organs were foregut, anterior midgut, posterior midgut, hindgut, Malpighian tubules, left salivary gland, right salivary gland, ventral nerve cord. Gene expression, transcription start and stop sites, and strands of termini were analysed. SMART amplification adapters were used for finding transcript termini and for phasing.
 
Overall design 3 embryos, 8 organs from each. PolyA RNA was reverse transcribed and amplified using an improved SMART protocol.
 
Contributor(s) Molnar D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 01, 2016
Last update date May 15, 2019
Contact name David Molnar
Organization name University of Cambridge
Department Genetics / Systems Biology Centre
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QR
Country United Kingdom
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (24)
GSM2051723 Animal 1: foregut
GSM2051724 Animal 2: foregut
GSM2051725 Animal 3: foregut
Relations
BioProject PRJNA310323
SRA SRP069131

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77429_RAW.tar 378.6 Mb (http)(custom) TAR (of BB, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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