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| Status |
Public on Feb 19, 2016 |
| Title |
Emergence of cephalosporin resitance in cephalosporin-sensitive class A penicillin binding protein mutants in Enterococcus faecium |
| Organism |
Enterococcus faecium |
| Experiment type |
Expression profiling by array
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| Summary |
E. faecium is inherantly resistant to cephalosporins. Resistance is lost in Class A penicillin binding protein PbfF PonA mutants, but is reversible by pencillin exposure.
E. faecium Affymetrix GeneChips were used to compare E. faecium expression properties of pbfF ponA mutant cells in the absence or presence of penicillin exposure.
Significant differences were observed between the expression properties of mock and penicillin treated E. faecium CV571 (pbfF ponA double mutant) cells.
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| Overall design |
E. faecium strain CV571 (pbfF ponA) were grown in laboratory medium to exponential under aerobic growth conditions. Cells were either mock treated or treated with penicillin for 30 min, total bacterial RNA was isolated, labeled and applied to Affymetrix GeneChips. We sought to determine whether the transcriptional profiles of E. faecium pbfF ponA mutant cells of penicillin challenged cells differed from that of mock treated CV571 cells.
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| Contributor(s) |
Dunman PM, Rice L |
| Citation(s) |
27048803 |
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| Submission date |
Feb 01, 2016 |
| Last update date |
May 06, 2016 |
| Contact name |
Paul Dunman |
| E-mail(s) |
paul_dunman@urmc.rochester.edu
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| Phone |
585-276-5700
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| Organization name |
University of Rochester
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| Department |
Microbiology and Immunology
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| Street address |
601 Elmwood Avenue
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL21391 |
Affymetrix Enterococcus faecium GeneChip [PMDefcma520788F] |
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| Samples (6)
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| GSM2051827 |
strain CV571, penicillin treatment, rep 1 |
| GSM2051828 |
strain CV571, penicillin treatment, rep 2 |
| GSM2051829 |
strain CV571, penicillin treatment, rep 3 |
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| Relations |
| BioProject |
PRJNA310329 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77436_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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